Document Detail


Dipeptide metabolism in the isolated perfused rat small intestine.
MedLine Citation:
PMID:  16839952     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The purpose of the present study was to investigate the utilisation of vascularly administered leucyl-leucine (Leu-Leu), alanyl-glutamine (Ala-Gln) and glycyl-glutamine (Gly-Gln) by the isolated vascularly perfused rat small intestine. Fractional extraction rates were 49%, 35.5%, and 12% for Leu-Leu, Ala-Gln, and Gly-Gln (0.15mM) corresponding to a net uptake of -63.5, -31.5, and -17 nmol/min/g wet weight. Nitrogen metabolism in terms of glutamine uptake and release of alanine and ammonia was not different when perfusion with dipeptides or with free amino-acids were compared. No soluble dipeptidase activity was released into the plasma- and cell-free synthetic vascular perfusate. No dipeptide could be recovered from the luminal perfusate. Considering the high fractional extraction rate for Leu-Leu, it is conceivable that dipeptide assimilation may occur also in extramucosal gut tissue. Although dipeptide transport cannot be excluded, dipeptide assimilation in small intestine may involve membrane bound peptidase(s), as in liver.
Authors:
M Plauth; I Kremer; A Raible; P Stehle; P Fürst; F Hartmann
Publication Detail:
Type:  Editorial    
Journal Detail:
Title:  Clinical nutrition (Edinburgh, Scotland)     Volume:  10 Suppl     ISSN:  0261-5614     ISO Abbreviation:  Clin Nutr     Publication Date:  1991  
Date Detail:
Created Date:  2006-07-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8309603     Medline TA:  Clin Nutr     Country:  England    
Other Details:
Languages:  eng     Pagination:  25-32     Citation Subset:  -    
Affiliation:
Eberhard-Karls-Universität, Medizinische Klinik und Poliklinik, Abteilung Innere Medizin I, D-7400 Tübingen, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Measurement of albumin synthesis in perfused rat liver using stable isotopes: [15N] hippurate as a m...
Next Document:  Role of membrane transport in the regulation of skeletal muscle glutamine turnover.