Document Detail

Dipeptide forms of glycine support mouse preimplantation embryo development in vitro and provide protection against high media osmolality.
MedLine Citation:
PMID:  22246224     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To examine potential benefits of dipeptide forms of amino acids for embryo culture by determining ability of dipeptide glycine forms to support embryo development, act as osmolytes, and reduce ammonia production.
METHODS: Frozen thawed 1-cell mouse embryos were cultured in media with varying osmolality with glycine and dipeptide forms of glycine and development assessed. Ammonia levels were measured in various media.
RESULTS: Dipeptide forms of glycine, alanyl- and glycyl-glycine, can support mouse embryo development in vitro. Additionally, dipeptide glycine can act as an organic osmolyte in developing embryos, permitting blastocyst formation in high osmolality media. Interestingly, as evidenced by decreased embryo development, dipeptides are not as efficient as osmolytes as their constituent individual amino acids. Dipeptide glycine produced less ammonia than glycine.
CONCLUSION: Though dipeptides can provide osmoregulation in preimplantation embryos, efficacy may be lower than individual amino acids. The mechanism by which embryos transport and utilize dipeptide amino acids remains to be identified.
Molly Moravek; Senait Fisseha; Jason E Swain
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Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2012-01-14
Journal Detail:
Title:  Journal of assisted reproduction and genetics     Volume:  29     ISSN:  1573-7330     ISO Abbreviation:  J. Assist. Reprod. Genet.     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-02-28     Completed Date:  2012-07-03     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  9206495     Medline TA:  J Assist Reprod Genet     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  283-90     Citation Subset:  IM    
Department of OB/GYN, Reproductive Sciences Program, University of Michigan, Ann Arbor, MI 48108, USA.
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MeSH Terms
Ammonia / metabolism
Blastocyst / metabolism*
Cell Count
Crosses, Genetic
Dipeptides / metabolism*
Embryo Culture Techniques
Glycine / analogs & derivatives,  metabolism*
Glycylglycine / metabolism
Osmolar Concentration
Water-Electrolyte Balance*
Reg. No./Substance:
0/Dipeptides; 556-50-3/Glycylglycine; 56-40-6/Glycine; 687-69-4/alanylglycine; 7664-41-7/Ammonia

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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