Document Detail

Dioxygenase and peroxidase activities of soybean lipoxygenase: synergistic interaction between linoleic acid and hydrogen peroxide.
MedLine Citation:
PMID:  2513625     Owner:  NLM     Status:  MEDLINE    
The interaction of H2O2 with soyabean lipoxygenase was investigated in the presence of linoleic acid. Dioxygenase activity was significantly higher at pH 9.0 than at pH 6.0. H2O2 at concentrations less than 1.0 nM stimulated linoleic acid oxidation synergistically and the magnitude of synergism was higher at pH 9.0. Linoleic acid dependent peroxidase activity towards benzidine, guaiacol, tetramethylbenzidine (TMBD) and tetramethylphenylenediamine (TMPD) was higher at pH 9.0, whereas pyrogallol and ABTS oxidation rates were higher at pH 6.0. H2O2 supported oxidation of benzidine, guaiacol, pyrogallol and ABTS was higher at pH 6.0, whereas TMPD, TMBD exhibited higher oxidation rates at pH 9.0. H2O2 in the presence of linoleic acid produced synergism in xenobotic metabolism and depending upon the substrate in question upto 11-fold increase in oxidation rate was noted.
A P Kulkarni; J Chaudhuri; A Mitra; I S Richards
Related Documents :
16342805 - Determination of pka values by capillary zone electrophoresis with a dynamic coating pr...
12807465 - Employment of stressful conditions during culture production to enhance subsequent cold...
24113545 - Eicosanoid post-mortem induction in kidney tissue is prevented by microwave irradiation.
24092895 - Anti-glutamic acid decarboxylase antibody associated limbic encephalitis in a child: ex...
717875 - Inhibition of calcium absorption in ponies fed diets containing oxalic acid.
19651625 - The position of the acid pocket as a major risk factor for acidic reflux in healthy sub...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Research communications in chemical pathology and pharmacology     Volume:  66     ISSN:  0034-5164     ISO Abbreviation:  Res. Commun. Chem. Pathol. Pharmacol.     Publication Date:  1989 Nov 
Date Detail:
Created Date:  1990-01-31     Completed Date:  1990-01-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0244734     Medline TA:  Res Commun Chem Pathol Pharmacol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  287-96     Citation Subset:  IM    
Florida Toxicology Research Center, College of Public Health, University of South Florida, Tampa 33612.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Drug Synergism
Hydrogen Peroxide / pharmacology*
Linoleic Acids / pharmacology*
Lipoxygenase / metabolism*
Oxygenases / metabolism
Peroxidases / metabolism
Soybeans / enzymology*
Reg. No./Substance:
0/Linoleic Acids; 7722-84-1/Hydrogen Peroxide; EC 1.11.1.-/Peroxidases; EC 1.13.-/Oxygenases; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  [Stability of the mixture of a new fat emulsion with parenteral nutrition solutions].
Next Document:  Variability of tracheobronchial clearance in healthy non-smoking subjects.