| Diosgenin induces apoptosis in IGF-1-stimulated human thyrocytes through two caspase-dependent pathways. | |
| | |
MedLine Citation:
|
PMID: 22266324 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Insulin-like growth factor-1 (IGF-1) is a growth factor of the thyroid that has been shown in our previous study to possess proliferative and antiapoptotic effects in FRTL-5 cell lines through the upregulation of cyclin D and Fas-associated death domain-like interleukin-1-converting enzyme (FLICE)-inhibitory protein (FLIP). Diosgenin, a natural steroid sapogenin from plants, has been shown to induce apoptosis in many cell lines, with the exception of thyroid cells. In this report, we investigated the apoptotic effect and mechanism of diosgenin in IGF-1-stimulated primary human thyrocytes. Primary human thyrocytes were preincubated with or without IGF-1 for 24h and subsequently exposed to varying concentrations of diosgenin for different times. We found that diosgenin induced apoptosis in human thyrocytes pretreated with IGF-1 in a dose-dependent manner through the activation of caspase cascades. Moreover, diosgenin inhibited FLIP and activated caspase-8 in the FAS-related apoptotic pathway. Diosgenin increased the production of ROS, regulated the balance of Bax and Bcl-2 and cleaved caspase-9 in the mitochondrial apoptotic pathway. These results indicate that diosgenin induces apoptosis in IGF-1-stimulated primary human thyrocytes through two caspase-dependent pathways. |
| | |
Authors:
|
Shumin Mu; Xingsong Tian; Yongwei Ruan; Yuantao Liu; Dezhi Bian; Chunyan Ma; Chunxiao Yu; Mei Feng; Furong Wang; Ling Gao; Jia-Jun Zhao |
Related Documents
:
|
11173974 - Ethanol and oxidative mechanisms in the brain. 9464644 - Tumor necrosis factor alpha augments amyloid beta protein (25-35) induced apoptosis in ... 21737934 - Isoegomaketone induces apoptosis through caspase-dependent and caspase-independent path... 21675854 - Tumor necrosis factor-α in liver ischemia/reperfusion injury. 9714 - Disseminated systemic expression of the "local" popliteal lymph node assay in rats. 22608254 - The immunobiology of il-27. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-1-11 |
Journal Detail:
|
Title: Biochemical and biophysical research communications Volume: - ISSN: 1090-2104 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
|
Created Date: 2012-1-23 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2012. Published by Elsevier Inc. |
Affiliation:
|
Provincial Hospital Affiliated to Shandong University, Jinan 250021, China; Hospital Affiliated to Shandong Traditional Chinese Medicine University, Jinan 250011, China; Institute of Endocrinology, Shandong Academy of Clinical Medicine, Jinan 250021, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: N-terminal acetyltransferase 3 gene is essential for robust circadian rhythm of bioluminescence repo...
Next Document: N-arachidonoyl glycine induces macrophage apoptosis via GPR18.