Document Detail

Diosgenin contents and DNA fingerprint screening of various yam (Dioscorea sp.) genotypes.
MedLine Citation:
PMID:  17294697     Owner:  NLM     Status:  MEDLINE    
In addition to the importance of many Dioscorea species (yams) as starchy staple food, some representatives are known and still used as a source for the steroidal sapogenin diosgenin, which, besides phytosterols derived from tall-oil, is an important precursor for partial synthesis of steroids for pharmaceutical research and applications. While in edible yams the diosgenin content should be as low as possible, a high yield of the compound is preferable for cultivars which are grown for the extraction of sterols. In the past, miscalculations and insufficiently precise techniques for quantification of diosgenin prevailed. Therefore we set out to re-evaluate the steroid content of a world collection of Dioscorea species, using leaves as sample material. We optimized diosgenin quantification techniques and fingerprinted the whole collection with the DNA amplification fingerprinting (DAF) technique. Total diosgenin contents ranged from 0.04 to 0.93% of dry weight within the collection. Several Dioscorea cultivars can be characterized via their DAF fingerprint patterns.
Oliver Vendl; Christoph Wawrosch; Christian Noe; Carlos Molina; Günter Kahl; Brigitte Kopp
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Zeitschrift für Naturforschung. C, Journal of biosciences     Volume:  61     ISSN:  0939-5075     ISO Abbreviation:  Z. Naturforsch., C, J. Biosci.     Publication Date:    2006 Nov-Dec
Date Detail:
Created Date:  2007-02-13     Completed Date:  2007-03-06     Revised Date:  2009-11-04    
Medline Journal Info:
Nlm Unique ID:  8912155     Medline TA:  Z Naturforsch C     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  847-55     Citation Subset:  IM    
Department of Pharmacognosy, University of Vienna, Althanstr. 14, A-1090 Vienna, Austria.
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MeSH Terms
DNA, Plant / genetics*,  isolation & purification
Dioscorea / chemistry*,  genetics*
Diosgenin / analysis*
Genetic Variation
Reg. No./Substance:
0/DNA, Plant; 512-04-9/Diosgenin

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