Document Detail


Diol appended quenchers for fluorescein boronic acid.
MedLine Citation:
PMID:  20127786     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Fluorescein isothiocyanate is treated with 3-aminophenylboronic acid to provide a fluorescently tagged boronic acid derivative which is used to assess Förster resonance energy transfer (FRET) quenching upon boronate ester formation with a series of bespoke diol appended quenchers. Fluorescence spectroscopy comparison of quenching efficiency between treatment of fluorescein and its boronic acid appended congener with quencher appended diol reveals boronate ester formation (covalently linked) to be the more efficient regime and from the panel of quenchers which also included nucleosides.
Authors:
Souad A Elfeky; Stephen E Flower; Naoko Masumoto; François D'Hooge; Ludivine Labarthe; Wenbo Chen; Christophe Len; Tony D James; John S Fossey
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Chemistry, an Asian journal     Volume:  5     ISSN:  1861-471X     ISO Abbreviation:  Chem Asian J     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-06-22     Completed Date:  2010-09-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101294643     Medline TA:  Chem Asian J     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  581-8     Citation Subset:  -    
Affiliation:
School of Chemistry, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.
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