Document Detail

Dimorphic nucleolar organizer regions in the frog Rana blairi.
MedLine Citation:
PMID:  67882     Owner:  NLM     Status:  MEDLINE    
The nucleolar organizer-specific staining procedure, ammoniacal silver (Ag-AS), has been used to study the distribution and size of the nucleolar organizer regions (NORs) in chromosomes of the frog Rana blairi (Mecham, Littlejohn, Oldham, Brown and Brown). The somatic metaphase karyotype of this frog is similar to that of other frogs of the Rana pipiens species complex, numerically (2n=26) and morphologically. Secondary constrictions are detectable in untreated Giemsa-stained metaphase preparations as achromatic gaps in the long arms of a pair of submetacentric chromosomes (no. 10). These constrictions are the only regions which are deeply stained with the Ag-AS method and are thus identified as the nucleolar organizer regions (Ag-NORs). In each of the three individuals, the Ag-NORs as visualized on the homologues are of unequal length.
O G Ward
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Canadian journal of genetics and cytology. Journal canadien de génétique et de cytologie     Volume:  19     ISSN:  0008-4093     ISO Abbreviation:  Can. J. Genet. Cytol.     Publication Date:  1977 Mar 
Date Detail:
Created Date:  1977-07-18     Completed Date:  1977-07-18     Revised Date:  2007-07-24    
Medline Journal Info:
Nlm Unique ID:  0372706     Medline TA:  Can J Genet Cytol     Country:  CANADA    
Other Details:
Languages:  eng     Pagination:  51-7     Citation Subset:  IM    
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MeSH Terms
Azure Stains
Cell Nucleolus / ultrastructure*
Chromosomes / ultrastructure*
Staining and Labeling
Reg. No./Substance:
0/Azure Stains; 7440-22-4/Silver

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