Document Detail


Diminished protein binding capacity of uremic sera for valproate following hemodialysis: role of free fatty acids and uremic compounds.
MedLine Citation:
PMID:  8739287     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We previously reported higher free phenytoin concentration in predialysis serum compared to postdialysis serum. In our present study with valproic acid, we observed an opposite effect, where free valproic acid concentration was higher in postdialysis serum. The increased free valproic acid concentrations in postdialysis sera (15.4-77.4% higher in postdialysis sera) were probably related to a 2-to 4-fold increase in free fatty acid concentrations in postdialysis sera. On the other hand, when an aliquot of predialysis serum pool was subjected to in vitro equilibrium dialysis and then both undialyzed and dialyzed serum pools were supplemented with valproic acid and incubated, the concentration of valproic acid in dialyzed serum was significantly lower than the undialyzed serum. This is probably related to removal of some uremic compounds during in vitro dialysis. Treatment of both pre- and postdialysis sera with activated charcoal corrected the binding deficiency of valproic acid due to removal of both uremic compounds and free fatty acids.
Authors:
A Dasgupta; M Jacques; D Malhotra
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  American journal of nephrology     Volume:  16     ISSN:  0250-8095     ISO Abbreviation:  Am. J. Nephrol.     Publication Date:  1996  
Date Detail:
Created Date:  1996-10-09     Completed Date:  1996-10-09     Revised Date:  2007-02-14    
Medline Journal Info:
Nlm Unique ID:  8109361     Medline TA:  Am J Nephrol     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  327-33     Citation Subset:  IM    
Affiliation:
Department of Pathology, University of New Mexico School of Medicine, Albuquerque, USA.
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MeSH Terms
Descriptor/Qualifier:
Anticonvulsants / blood*
Charcoal
Creatinine / blood
Fatty Acids, Nonesterified / blood*
Humans
Protein Binding
Renal Dialysis*
Serum Albumin / metabolism*
Urea / blood
Uremia / blood*,  therapy
Valproic Acid / blood*
Chemical
Reg. No./Substance:
0/Anticonvulsants; 0/Fatty Acids, Nonesterified; 0/Serum Albumin; 16291-96-6/Charcoal; 57-13-6/Urea; 60-27-5/Creatinine; 99-66-1/Valproic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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