Document Detail

Diminished interleukin-8 (IL-8) production in the fetal wound healing response.
MedLine Citation:
PMID:  9698538     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Fetal skin wound healing results in scarless repair with minimal cellular inflammatory response. Interleukin-8 (IL-8) stimulates inflammation in postnatal wound healing but little is known about its role in fetal wounds. We hypothesized that fetal tissues have diminished IL-8 during wound repair and in response to platelet-derived growth factor (PDGF), a growth factor central to wound healing. MATERIALS AND METHODS: To examine the IL-8 response of fibroblasts to PDGF, cultures of human fetal (17-18 weeks) and adult dermal fibroblasts were incubated 8 h with PDGF (0, 0.1, 1, or 10 ng/mL) and supernatants and cells were collected for IL-8 ELISA and IL-8 RT-PCR. To evaluate the IL-8 response to wounding, human adult and fetal skin was placed subcutaneously in the SCID mouse, wounded, and the wound cleft excised after 4, 12, 24, or 72 h for IL-8 RT-PCR. RESULTS: Fetal fibroblasts produced less IL-8 protein at baseline (50 +/- 6 pg/mL versus 450 +/- 115 pg/mL, P < 0.001) and in response to all concentrations of PDGF examined (P < 0.001). IL-8 mRNA was detected in unstimulated adult fibroblasts but not in fetal fibroblasts. Much less IL-8 mRNA was detected in stimulated fetal fibroblasts than in adult fibroblasts. IL-8 mRNA was detected 4 h after wounding in fetal and adult wounds. By 12 h no IL-8 mRNA was detected in fetal wounds, whereas adult wounds had IL-8 mRNA persisting to 72 h. CONCLUSIONS: Diminished inflammatory cytokine response by fetal tissues may be responsible for the lack of cellular recruitment and inflammation seen in fetal wound healing and may contribute to scarless wound repair.
K W Liechty; T M Crombleholme; D L Cass; B Martin; N S Adzick
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of surgical research     Volume:  77     ISSN:  0022-4804     ISO Abbreviation:  J. Surg. Res.     Publication Date:  1998 Jun 
Date Detail:
Created Date:  1998-09-04     Completed Date:  1998-09-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376340     Medline TA:  J Surg Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  80-4     Citation Subset:  IM    
Copyright Information:
Copyright 1998 Academic Press.
Department of Surgery, The Children's Hospital of Philadelphia, and the University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, 19104, USA.
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MeSH Terms
Aging / physiology
Cells, Cultured
Fetus / metabolism*
Fibroblasts / metabolism
Interleukin-8 / biosynthesis*,  genetics
Mice, SCID
Platelet-Derived Growth Factor / pharmacology
Polymerase Chain Reaction
RNA, Messenger / metabolism
Skin / embryology*,  injuries*,  pathology
Time Factors
Transcription, Genetic
Wound Healing / physiology*
Reg. No./Substance:
0/Interleukin-8; 0/Platelet-Derived Growth Factor; 0/RNA, Messenger

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