| Diminished interleukin-8 (IL-8) production in the fetal wound healing response. | |
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MedLine Citation:
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PMID: 9698538 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Fetal skin wound healing results in scarless repair with minimal cellular inflammatory response. Interleukin-8 (IL-8) stimulates inflammation in postnatal wound healing but little is known about its role in fetal wounds. We hypothesized that fetal tissues have diminished IL-8 during wound repair and in response to platelet-derived growth factor (PDGF), a growth factor central to wound healing. MATERIALS AND METHODS: To examine the IL-8 response of fibroblasts to PDGF, cultures of human fetal (17-18 weeks) and adult dermal fibroblasts were incubated 8 h with PDGF (0, 0.1, 1, or 10 ng/mL) and supernatants and cells were collected for IL-8 ELISA and IL-8 RT-PCR. To evaluate the IL-8 response to wounding, human adult and fetal skin was placed subcutaneously in the SCID mouse, wounded, and the wound cleft excised after 4, 12, 24, or 72 h for IL-8 RT-PCR. RESULTS: Fetal fibroblasts produced less IL-8 protein at baseline (50 +/- 6 pg/mL versus 450 +/- 115 pg/mL, P < 0.001) and in response to all concentrations of PDGF examined (P < 0.001). IL-8 mRNA was detected in unstimulated adult fibroblasts but not in fetal fibroblasts. Much less IL-8 mRNA was detected in stimulated fetal fibroblasts than in adult fibroblasts. IL-8 mRNA was detected 4 h after wounding in fetal and adult wounds. By 12 h no IL-8 mRNA was detected in fetal wounds, whereas adult wounds had IL-8 mRNA persisting to 72 h. CONCLUSIONS: Diminished inflammatory cytokine response by fetal tissues may be responsible for the lack of cellular recruitment and inflammation seen in fetal wound healing and may contribute to scarless wound repair. |
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Authors:
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K W Liechty; T M Crombleholme; D L Cass; B Martin; N S Adzick |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of surgical research Volume: 77 ISSN: 0022-4804 ISO Abbreviation: J. Surg. Res. Publication Date: 1998 Jun |
Date Detail:
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Created Date: 1998-09-04 Completed Date: 1998-09-04 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0376340 Medline TA: J Surg Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 80-4 Citation Subset: IM |
Copyright Information:
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Copyright 1998 Academic Press. |
Affiliation:
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Department of Surgery, The Children's Hospital of Philadelphia, and the University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, 19104, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aging / physiology Animals Cells, Cultured Fetus / metabolism* Fibroblasts / metabolism Humans Interleukin-8 / biosynthesis*, genetics Mice Mice, SCID Platelet-Derived Growth Factor / pharmacology Polymerase Chain Reaction RNA, Messenger / metabolism Skin / embryology*, injuries*, pathology Time Factors Transcription, Genetic Wound Healing / physiology* |
| Chemical | |
Reg. No./Substance:
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0/Interleukin-8; 0/Platelet-Derived Growth Factor; 0/RNA, Messenger |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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