| Diminished inibitory action of ethanol on the contraction of gallbladder isolated from chronically ethanol-fed Guinea pigs. | |
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MedLine Citation:
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PMID: 11901296 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Ethanol is known to decrease the gallbladder contractility. The purpose of this study was to clarify the mechanism of tolerance to the inhibitory action of ethanol on the gallbladder contractility. Male guinea pigs were fed ethanol (3%) or calorie-matched sucrose in the drinking water for 4 weeks. Then, the gallbladder was isolated, and its isometric tension was measured. The contractile responses to KCl, BAY K8644, histamine, and phorbol 12,13-dibutyrate in the normal medium were not different between the gallbladder strips from ethanol-fed and control guinea pigs. Ethanol at 25 mmol/l in vitro did not affect the contractile responses to KCl and BAY K8644 in the gallbladder strips from both ethanol-fed and control guinea pigs. On the other hand, ethanol at 25 mmol/l in vitro significantly inhibited the contractile responses to histamine and phorbol 12,13-dibutyrate in the gallbladder strips from the control guinea pigs, but it did not affect the contractile response to histamine and significantly augmented that to phorbol 12,13-dibutyrate in the strips from the ethanol-fed guinea pigs. Diphenhydramine, a selective H(1) receptor antagonist, abolished the histamine contraction in gallbladder strips from both control and ethanol-fed guinea pigs, while cimetidine, a selective H(2) receptor antagonist, did not affect histamine contraction, implying that histamine-induced contraction of guinea pig gallbladders is mediated only by H(1) receptors. Verapamil (1 micromol/l) completely inhibited the phorbol 12,13-dibutyrate-induced contraction of the strips from both ethanol-fed and control guinea pigs. The histamine-induced contraction was partly inhibited in the absence of Ca(2+) in the medium. In the gallbladder strips from both ethanol-fed and control guinea pigs, ethanol at 25 mmol/ in vitro did not affect the histamine-induced contraction in the absence of extracellular Ca(2+). Tolerance to the inhibitory action of ethanol developed selectively on contractile responses to histamine and phorbol 12,13-dibutyrate. Chronic ethanol administration produces tolerance to in vitro gallbladder contractility mediated by the Ca(2+) entry through L-type Ca(2+) channels linked with protein kinase C activation. |
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Authors:
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H Masui; I Wakabayashi; K Siogawa; N Koizumi |
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Publication Detail:
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Type: Comparative Study; In Vitro; Journal Article |
Journal Detail:
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Title: Pharmacology Volume: 65 ISSN: 0031-7012 ISO Abbreviation: Pharmacology Publication Date: 2002 May |
Date Detail:
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Created Date: 2002-03-19 Completed Date: 2002-08-13 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0152016 Medline TA: Pharmacology Country: Switzerland |
Other Details:
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Languages: eng Pagination: 10-7 Citation Subset: IM |
Copyright Information:
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Copyright 2002 S. Karger AG, Basel |
Affiliation:
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Department of Public Health, Hyogo College of Medicine, Nishinomiya, Japan. masui11@hyg-gw.hyo-med.ac.jp |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester
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pharmacology Animals Body Weight / drug effects Calcium Channel Agonists / pharmacology Dose-Response Relationship, Drug Ethanol / administration & dosage, pharmacology* Gallbladder / drug effects*, physiology Guinea Pigs Histamine / pharmacology Male Muscle Contraction / drug effects* Phorbol 12,13-Dibutyrate / pharmacology Potassium Chloride / pharmacology Verapamil / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Calcium Channel Agonists; 37558-16-0/Phorbol 12,13-Dibutyrate; 51-45-6/Histamine; 52-53-9/Verapamil; 64-17-5/Ethanol; 71145-03-4/3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester; 7447-40-7/Potassium Chloride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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