Document Detail


Dimethyl sulfoxide enhances polyethylene glycol-mediated somatic cell fusion.
MedLine Citation:
PMID:  1028173     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The efficiency of fusion of human diploid cells by polyethylene glycol was greatly enhanced by addition of dimethyl sulfoxide. The extent of fusion was directly proportional to the concentrations of both of these compounds. At all except the highest concentrations, cell loss was moderate to minimal and perturbation of cell cycle function as measured by [3H] thymidine labeling indices and mitotic indices was minimal in the surviving cells. This technique is potentially useful for heterokaryon studies as well as for the isolation of hybrids of mammalian somatic cells.
Authors:
T H Norwood; C J Zeigler; G M Martin
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Somatic cell genetics     Volume:  2     ISSN:  0098-0366     ISO Abbreviation:  Somatic Cell Genet.     Publication Date:  1976 May 
Date Detail:
Created Date:  1977-09-02     Completed Date:  1977-09-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7506054     Medline TA:  Somatic Cell Genet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  263-70     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Fusion / drug effects*
Dimethyl Sulfoxide / pharmacology*
Dose-Response Relationship, Drug
Humans
Polyethylene Glycols / pharmacology*
Stimulation, Chemical
Chemical
Reg. No./Substance:
0/Polyethylene Glycols; 67-68-5/Dimethyl Sulfoxide

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