Document Detail


Dimethyl amiloride, a Na+-H+ exchange inhibitor, and its cardioprotective effects in hemorrhagic shock in in vivo resuscitated rats.
MedLine Citation:
PMID:  19340541     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Stimulation of the Na(+)-H(+) exchanger plays an important role in the pathway of myocardial dysfunction and injury following hemorrhagic shock. Inhibition of the Na(+)-H(+) exchanger appears to be a new pharmacological tool for myocardial protection. Despite the extensive research that has been done on the role of the Na(+)-H(+) exchanger in ischemia reperfusion, little is known about the role of the exchanger following hemorrhagic shock. The purpose of this study was to examine the protective effects of blocking the cardiac Na(+)-H(+) exchanger, using 20 microM dimethyl amiloride (DMA), a specific Na(+)-H(+) exchanger blocker, on myocardial contractile function after ex vivo perfusion of isolated rat heart following 1 h of hemorrhagic shock. Sprague-Dawley rats were assigned to hemorrhage + DMA, hemorrhage, sham hemorrhage + DMA and sham hemorrhage groups (n = 6 per group). Hearts were perfused with a balanced salt solution for 60 min. In the DMA treated group, 20 microM DMA was added for the first 5 min of the 60-min ex vivo heart resuscitation. The results showed that inhibition of the Na(+)-H(+) exchanger for 5 min on ex vivo perfusion of the isolated hearts following hemorrhagic shock using 20 microM DMA improved myocardial contractile function. Blocking the Na(+)-H(+) exchanger on ex vivo perfusion of isolated hearts using 20 muM DMA has protective effects on myocardial contractile function.
Authors:
Mona Soliman
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-03-07
Journal Detail:
Title:  The journal of physiological sciences : JPS     Volume:  59     ISSN:  1880-6562     ISO Abbreviation:  J Physiol Sci     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-04-15     Completed Date:  2010-01-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101262417     Medline TA:  J Physiol Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  175-80     Citation Subset:  IM    
Affiliation:
Department of Physiology, College of Medicine, King Khalid University Hospital, P.O. Box 2925 (29), Riyadh, 11461, Saudi Arabia. monaslmn@yahoo.com
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MeSH Terms
Descriptor/Qualifier:
Amiloride / analogs & derivatives*,  pharmacology,  therapeutic use
Animals
Dose-Response Relationship, Drug
Heart Failure / physiopathology,  prevention & control*
Male
Models, Animal
Myocardial Contraction / drug effects,  physiology
Rats
Rats, Sprague-Dawley
Resuscitation / adverse effects*
Shock, Hemorrhagic / complications*,  physiopathology
Sodium-Hydrogen Antiporter / antagonists & inhibitors*
Time Factors
Chemical
Reg. No./Substance:
0/Sodium-Hydrogen Antiporter; 1214-79-5/5-dimethylamiloride; 2609-46-3/Amiloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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