Document Detail


Dimeric surfactant protein B peptide sp-b(1-25) in neonatal and acute respiratory distress syndrome.
MedLine Citation:
PMID:  12490037     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Surfactant protein B (SP-B) is a constituent surfactant protein critical for normal lung function. Monomeric SP-B(1-25) (mSP-B(1-25)), a peptide based on the N-terminal domain of human SP-B, mixed in phospholipids partially restores lung function in surfactant-deficient animals. Because native SP-B is a homodimer, we synthesized and tested dimeric SP-B(1-25) (dSP-B(1-25)). Circular dichroism (CD) and Fourier Transform Infrared (FTIR) spectroscopy indicated that the secondary conformation of SP-B(1-25) was not significantly perturbed by dimerization. The effects on lung function were compared to phospholipids and Survanta in models of neonatal respiratory distress syndrome (RDS) and acute RDS (ARDS). Preterm rabbits born at 27 days of gestation received 100 mg surfactant / kg at birth and were ventilated for 1 hour with a tidal volume of 10 mL / kg. Dynamic compliance was monitored every 15 minutes and postmortem pressure-volume curves were measured. Adult rats were lavaged to induce surfactant deficiency, treated with 100 mg surfactant / kg, and ventilated for 2 hours. Lung function was assessed using arterial blood gases and dynamic compliance every 15 minutes and postmortem pressure-volume curves. Lung volumes in both models and oxygenation in the lavaged rats were consistently higher in the dSP-B(1-25) than in the Survanta and mSP-B(1-25) surfactant groups. The data suggest that dSP-B(1-25) is more efficient in restoring lung function in neonatal RDS and ARDS than mSP-B(1-25) surfactant.
Authors:
Frans J Walther; José M Hernandez-Juviel; Larry M Gordon; Mark A Sherman; Alan J Waring
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental lung research     Volume:  28     ISSN:  0190-2148     ISO Abbreviation:  Exp. Lung Res.     Publication Date:  2002 Dec 
Date Detail:
Created Date:  2002-12-19     Completed Date:  2003-05-09     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8004944     Medline TA:  Exp Lung Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  623-40     Citation Subset:  IM    
Affiliation:
Research and Education Institute at Harbor-UCLA Medical Center, Torrance, California 90502, USA. fjwalter@aol.com
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MeSH Terms
Descriptor/Qualifier:
Acute Disease
Adult
Amino Acid Sequence
Animals
Animals, Newborn
Circular Dichroism
Dimerization
Disease Models, Animal
Humans
Infant, Newborn
Molecular Sequence Data
Protein Structure, Tertiary
Pulmonary Surfactant-Associated Protein B / analysis,  chemistry*,  metabolism*
Rabbits
Rats
Respiration, Artificial
Respiratory Distress Syndrome, Adult / metabolism*
Respiratory Distress Syndrome, Newborn / metabolism*
Spectroscopy, Fourier Transform Infrared
Grant Support
ID/Acronym/Agency:
GM50483/GM/NIGMS NIH HHS; HL55534/HL/NHLBI NIH HHS; P30 CA33572/CA/NCI NIH HHS; S10 RR14857/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Pulmonary Surfactant-Associated Protein B

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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