Document Detail

Diluted isoflurane as a suitable alternative for diethyl ether for rat anaesthesia in regular toxicology studies.
MedLine Citation:
PMID:  18057828     Owner:  NLM     Status:  MEDLINE    
Despite its explosive properties and toxicity to both animals and humans, diethyl ether is an agent long used in Japan in the anaesthesia jar method of rat anaesthetises. However, in response to a recent report from the Science Council of Japan condemning diethyl ether as acceptable practice, we searched for an alternative rat anaesthesia method that provided data continuous with pre-existing regular toxicology studies already conducted under diethyl ether anaesthesia. For this, we examined two candidates; 30% isoflurane diluted with propylene glycol and pentobarbitone. Whereas isoflurane is considered to be one of the representatives of modern volatile anaesthetics, the method of propylene glycol-diluted 30% isoflurane used in this study was our modification of a recently reported method revealed to have several advantages as an inhalation anaesthesia. Intraperitoneal pentobarbitone has long been accepted as a humane method in laboratory animal anaesthesiology. These 2 modalities were scrutinized in terms of consistency of haematology and blood chemistry with previous results using ether. We found that pentobarbitone required a much longer induction time than diethyl ether, which is suspected to be the cause of fluctuations in several haematological and blood chemical results. Conversely, only calcium ion concentration showed a slight difference from traditional results in the case of 30% isoflurane. Additionally, serum prolactin and corticosterone levels indicated that 30% isoflurane induced less stress than ether, confirming that 30% isoflurane can both provide results consistent with diethyl ether, while at the same time remove its disadvantages. As such 30% isoflurane appears to be a strong alternative anaesthetic agent for future regular toxicology studies in Japan.
Toshiaki Nagate; Tomonobu Chino; Chizuru Nishiyama; Daisuke Okuhara; Toru Tahara; Yoshimasa Maruyama; Hiroko Kasahara; Kayoko Takashima; Sayaka Kobayashi; Yoshiyuki Motokawa; Shin-ichi Muto; Junji Kuroda
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of veterinary medical science / the Japanese Society of Veterinary Science     Volume:  69     ISSN:  0916-7250     ISO Abbreviation:  J. Vet. Med. Sci.     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-12-06     Completed Date:  2008-01-10     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9105360     Medline TA:  J Vet Med Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1137-43     Citation Subset:  IM    
Toxicology Research Laboratory, R&D, Kissei Pharmaceutical Co., Ltd, 2320-1 Maki, Hotaka, Azunmino-City, Nagano-Pref. 399-8305, Japan.
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MeSH Terms
Adjuvants, Anesthesia / pharmacology
Adrenocorticotropic Hormone / blood
Anesthesia / veterinary*
Anesthesia, Inhalation / veterinary*
Anesthetics, Inhalation / pharmacology
Dose-Response Relationship, Drug
Ether, Ethyl / pharmacology*
Isoflurane / administration & dosage,  pharmacology*
Pentobarbital / pharmacology
Rats, Sprague-Dawley
Stress, Physiological
Time Factors
Reg. No./Substance:
0/Adjuvants, Anesthesia; 0/Anesthetics, Inhalation; 26675-46-7/Isoflurane; 60-29-7/Ether, Ethyl; 76-74-4/Pentobarbital; 9002-60-2/Adrenocorticotropic Hormone

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