Document Detail


Dihydrotetramethylrosamine: a long wavelength, fluorogenic peroxidase substrate evaluated in vitro and in a model phagocyte.
MedLine Citation:
PMID:  2018489     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Dihydrotetramethylrosamine, a fluorogenic substrate for peroxidase, and its fluorescent oxidation product, tetramethylrosamine chloride, were evaluated. The substrate is colorless and nonfluorescent while the oxidized dye absorbs at 550 nm and emits at 574 nm in both methanol and water. In vitro assays demonstrated that the substrate was oxidized to the fluorophore by horseradish peroxidase in the presence of hydrogen peroxide. In vivo uptake and oxidation of the substrate by Amoeba proteus was characterized by the initial appearance of fluorescent phagocytic vacuoles with subsequent localization in vesicular organelles the size and shape of protozoan mitochondria. Similar staining patterns occurred in cells incubated with substrate, oxidized rosamine or rhodamine 123, a known mitochondrial stain.
Authors:
J E Whitaker; P L Moore; R P Haugland; R P Haugland
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  175     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1991 Mar 
Date Detail:
Created Date:  1991-05-23     Completed Date:  1991-05-23     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  387-93     Citation Subset:  IM    
Affiliation:
Molecular Probes, Inc., Eugene, OR 97402.
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MeSH Terms
Descriptor/Qualifier:
Amoeba / metabolism
Animals
Fluorescent Dyes*
Microscopy, Fluorescence
Oxidation-Reduction
Peroxidases / metabolism*
Phagocytes / metabolism*
Rhodamines* / metabolism
Spectrometry, Fluorescence
Spectrum Analysis
Grant Support
ID/Acronym/Agency:
GM37347/GM/NIGMS NIH HHS; GM38987/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; 0/Rhodamines; 105284-17-1/dihydrotetramethylrosamine; EC 1.11.1.-/Peroxidases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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