Document Detail


Dihydrotestosterone and bicalutamide do not affect periostin expression in androgen-dependent LNCaP prostate cancer cell lines.
MedLine Citation:
PMID:  23482749     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND/AIM: To investigate periostin (POSTN) expression in the LNCaP cell line.
MATERIALS AND METHODS: Our LNCaP strain did not constitutively express the POSTN gene. Through cell transfection with a cloning vector, we developed an LNCaP cell line that stably expressed POSTN. LNCaP wild-type and transfected cells were incubated with dihydrotestosterone (DHT) in the presence/or absence of bicalutamide (BIC). POSTN mRNA was detected by quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) and growth was measured with the MTT assay.
RESULTS: POSTN transfection stimulated LNCaP cell growth. While POSTN transfection did not interfere with the stimulatory effect of DHT, BIC had an inhibitory effect on cell proliferation. However, exposure to either DHT and/or BIC was not able to interfere with POSTN expression per se.
CONCLUSION: We confirmed the role of POSTN in promoting cancer cell growth. Although POSTN transcription is not likely to be androgen-dependent, the fact that increased cell proliferation POSTN-mediated was impaired by BIC suggests an androgen modulation of POSTN interaction proteins.
Authors:
Francesca Argellati; Pier Vitale Nuzzo; Francesco Ricci; Rosa Mangerini; Alessandra Rubagotti; Francesco Boccardo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Anticancer research     Volume:  33     ISSN:  1791-7530     ISO Abbreviation:  Anticancer Res.     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-03-13     Completed Date:  2013-05-16     Revised Date:  2013-05-31    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  815-20     Citation Subset:  IM    
Affiliation:
IRCCS San Martino University Hospital - IST National Cancer Research Institute and the University of Genoa, Academic Unit of Medical Oncology (UOC Oncologia Medica B), Largo Rosanna Benzi 10, 16132 Genoa, Italy. f.boccardo@unige.it
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MeSH Terms
Descriptor/Qualifier:
Androgens / physiology*
Anilides / pharmacology*
Cell Adhesion Molecules / genetics,  physiology*
Cell Line, Tumor
Cell Proliferation / drug effects
Dihydrotestosterone / pharmacology*
Humans
Male
Neoplasms, Hormone-Dependent / pathology*
Nitriles / pharmacology*
Prostatic Neoplasms / pathology*
RNA, Messenger / analysis
Tosyl Compounds / pharmacology*
Chemical
Reg. No./Substance:
0/Androgens; 0/Anilides; 0/Cell Adhesion Molecules; 0/Nitriles; 0/POSTN protein, human; 0/RNA, Messenger; 0/Tosyl Compounds; 521-18-6/Dihydrotestosterone; 90357-06-5/bicalutamide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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