Document Detail


Digestion of maize sphingolipids in rats and uptake of sphingadienine by Caco-2 cells.
MedLine Citation:
PMID:  12949364     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We investigated the digestion of cerebrosides of plant origin prepared from maize, focusing especially on the digestive fates of trans-4, cis-8- and trans-4, trans-8-sphingadienine, which are common in higher plants. In the small intestinal mucosa and cecal contents of rats, the cerebrosidase activity at pH 5.2 toward the glucosyl linkage in maize cerebrosides (glucosylceramides) was similar to that in cerebrosides of mammalian origin. Similarly, the ceramidase activity toward the amide linkage in ceramides prepared from maize cerebrosides at pH 7.0 was the same as that toward ceramides of mammalian origin. In addition, maize cerebrosides were hydrolyzed to ceramide and free sphingoid bases in the digestive tract of rats after oral administration. To further evaluate the uptake by enterocytes of 4,8-sphingadienine, we used differentiated Caco-2 cells, derived from human colonic carcinoma, as a model of intestinal epithelial cells. The accumulation of sphingoid bases in Caco-2 cells incubated with each isomer of sphingadienine was lower than that after incubation with sphingosine (P < 0.05). Verapamil, a P-glycoprotein inhibitor, increased the accumulation of each sphingadienine but not of sphingosine, suggesting that the efflux of sphingadienine of plant origin, but not sphingosine of mammalian origin, was affected by P-glycoprotein. The digestibility of maize cerebrosides appears similar to that of cerebrosides of mammalian origin, but the metabolic fate of sphingoid bases of plant origin within enterocytes differs from that of sphingosine. Isomers of 4,8-sphingadienine degraded from dietary plant cerebrosides appear to be poorly absorbed from the digestive tract.
Authors:
Tatsuya Sugawara; Mikio Kinoshita; Masao Ohnishi; Junichi Nagata; Morio Saito
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of nutrition     Volume:  133     ISSN:  0022-3166     ISO Abbreviation:  J. Nutr.     Publication Date:  2003 Sep 
Date Detail:
Created Date:  2003-09-01     Completed Date:  2003-10-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0404243     Medline TA:  J Nutr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2777-82     Citation Subset:  IM    
Affiliation:
Division of Food Science, Incorporated Administrative Agency, National Institute of Health and Nutrition, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8636, Japan. sugawara@nih.go.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Caco-2 Cells / metabolism
Digestion*
Ethanolamines / pharmacokinetics*
Humans
Hydrolysis
Intestines / metabolism
Male
Rats
Rats, Sprague-Dawley
Sphingolipids / metabolism*
Sphingosine / metabolism
Zea mays / chemistry*
Chemical
Reg. No./Substance:
0/Ethanolamines; 0/Sphingolipids; 123-78-4/Sphingosine; 25696-03-1/sphingadienine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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