Document Detail


Digalactoside expression in the lipopolysaccharide of Haemophilus influenzae and its role in intravascular survival.
MedLine Citation:
PMID:  16177385     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Digalactoside (galalpha-1-4 galbeta) structures of the lipopolysaccharide (LPS) of Haemophilus influenzae are implicated in virulence. A confounding factor is that tetranucleotide repeats within the lic2A, lgtC, and lex2 genes mediate phase-variable expression of the digalactosides. By deleting these repeats, we constructed recombinant strains of RM153 constitutively expressing either one or two LPS digalactosides. Expression of two digalactosides, rather than one, was associated with increased virulence of H. influenzae in vivo.
Authors:
Ruth Griffin; Chris D Bayliss; Mark A Herbert; Andrew D Cox; Katherine Makepeace; James C Richards; Derek W Hood; E Richard Moxon
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Infection and immunity     Volume:  73     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-09-22     Completed Date:  2005-11-28     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7022-6     Citation Subset:  IM    
Affiliation:
Molecular Infectious Diseases Group, University of Oxford Department of Paediatrics, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Headington, UK. r.griffin@imperial.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacteremia / microbiology*
Bacterial Proteins / genetics
Carbohydrate Sequence
Disaccharides / analysis,  metabolism*
Genes, Bacterial / genetics
Haemophilus Infections / microbiology*
Haemophilus influenzae / genetics*,  pathogenicity*
Lipopolysaccharides / biosynthesis*,  chemistry
Molecular Sequence Data
Rats
Rats, Sprague-Dawley
Sequence Deletion
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Disaccharides; 0/Lipopolysaccharides; 0/lic2A protein, Haemophilus influenzae; 13117-26-5/4-O-alpha-D-galactopyranosyl-D-galactose
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