| Difructose anhydride III promotes iron absorption in the rat large intestine. | |
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MedLine Citation:
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PMID: 20005467 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: We examined the intestinal segment responsible for and the mechanisms involved in the non-digestible disaccharide difructose anhydride III (DFAIII)-mediated enhancement of iron absorption. METHODS: Net iron and calcium absorption (percentage) were observed in male Sprague-Dawley rats fed an AIN93G-based diet with or without DFAIII (30 g/kg of diet) for 2 wk after cecectomy or laparotomy (experiment 1). The absorption rates of calcium and iron from ligated jejunal loops (experiment 2) or ligated cecal sacs (experiment 3) in anesthetized rats fed a control or DFA diet were observed with or without the addition of DFAIII to the instilled mucosal fluid. Levels of the major iron transporter divalent metal transporter-1 (DMT-1) mRNA per 28S rRNA were evaluated by real-time polymerase chain reaction in the intestinal mucosa. RESULTS: Net absorption (percentage) of iron and calcium was higher in the DFA group than in the DFA-free group in laparotomized rats. The enhancement of iron absorption was almost and calcium absorption was partly abolished by cecectomy. The absorption rate of calcium, but not of iron, from the jejunal loops was increased by the addition of DFAIII (100 mmol/L). Iron absorption was clearly higher in the ligated cecal sacs of the DFAIII-fed group than in the DFA-free group with or without the mucosal addition of DFAIII. DMT-1 mRNA level per 28S rRNA was maintained in the cecal mucosa through large increases in cecal size due to DFAIII feeding. CONCLUSION: The DFAIII-induced increases in iron absorption are the result of increased cecal iron absorptive capacity through expansion of the cecal mucosa maintaining DMT-1 mRNA expression. |
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Authors:
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Hiroshi Hara; Satoshi Onoshima; Chie Nakagawa |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Nutrition (Burbank, Los Angeles County, Calif.) Volume: 26 ISSN: 1873-1244 ISO Abbreviation: Nutrition Publication Date: 2010 Jan |
Date Detail:
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Created Date: 2009-12-16 Completed Date: 2010-04-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8802712 Medline TA: Nutrition Country: United States |
Other Details:
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Languages: eng Pagination: 120-7 Citation Subset: IM |
Affiliation:
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Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan. hara@chem.agr.hokudai.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Calcium, Dietary / pharmacokinetics* Cation Transport Proteins / genetics, metabolism Cecum / metabolism* Disaccharides / pharmacology* Intestinal Absorption / drug effects* Intestinal Mucosa / metabolism Iron, Dietary / pharmacokinetics* Jejunum / metabolism* Male RNA, Messenger / metabolism Rats Rats, Sprague-Dawley |
| Chemical | |
Reg. No./Substance:
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0/Calcium, Dietary; 0/Cation Transport Proteins; 0/Disaccharides; 0/Iron, Dietary; 0/RNA, Messenger; 0/solute carrier family 11- (proton-coupled divalent metal ion transporters), member 2; 81129-73-9/difructose anhydride III |
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