Document Detail


Diffusion through agar blocks of finite dimensions: a theoretical analysis of three systems of practical significance in microbiology.
MedLine Citation:
PMID:  10217498     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A number of experimental methods in biology depend on the kinetics of diffusion of a substance through a gel. This paper reviews the diffusion equations, gives the experimental limitations for some useful cases, and presents computer simulations for cases that cannot be treated analytically. While double diffusion is not considered, three single-diffusion situations are treated. (1) Systems for the study of chemotaxis in the gliding bacterium Myxococcus xanthus. Experimental designs used for this in many cases in the literature were inappropriate and mathematical analysis of these is presented. (2) The development of gradient plates. The time necessary for vertical diffusion to become substantially complete and before diffusion in the direction of the original slant has proceeded significantly is calculated. (3) The application to antimicrobial disk susceptibility tests. The basis of the measurement of antibiotic sensitivities with disks containing antimicrobial agents, as routinely used in clinical microbiological and testing laboratories, is analysed and the limitations are assessed and improvements suggested.
Authors:
A L Koch
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Microbiology (Reading, England)     Volume:  145 ( Pt 3)     ISSN:  1350-0872     ISO Abbreviation:  Microbiology (Reading, Engl.)     Publication Date:  1999 Mar 
Date Detail:
Created Date:  1999-06-15     Completed Date:  1999-06-15     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9430468     Medline TA:  Microbiology     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  643-54     Citation Subset:  IM    
Affiliation:
Biology Department, Indiana University, Bloomington 47405-6801, USA. Koch@Indiana.edu
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MeSH Terms
Descriptor/Qualifier:
Agar / chemistry*
Chemotaxis
Computer Simulation*
Diffusion*
Gels / chemistry
Microbial Sensitivity Tests
Microbiology
Models, Theoretical*
Myxococcus xanthus / physiology
Grant Support
ID/Acronym/Agency:
RR703126/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Gels; 9002-18-0/Agar

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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