| Diffusion of singlet oxygen into human bronchial epithelial cells. | |
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MedLine Citation:
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PMID: 3030447 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The respiratory epithelium undergoes morphological and functional changes following exposure to single oxygen. However, mechanisms by which singlet oxygen causes cellular injury are unclear. The present experiments were designed to investigate the possibility that singlet oxygen, a highly reactive species, diffuses into respiratory epithelial cells. Of the various methods for detection of singlet oxygen, the electron spin resonance (ESR) spectrometric technique was judged to be most compatible and sensitive for use with cell suspensions. ESR spectrometry was used to monitor the singlet oxygen-mediated conversion of 2-(9,10-dimethoxyanthracenyl)-tert-butylhydroxylamine, (I), to 2-(9,10-dimethoxyanthracenyl)-tert-butylnitroxide, (II), and its corresponding endoperoxide, (III), in human bronchial epithelial cells treated with extracellularly generated singlet oxygen. In a second series of experiments, bronchial epithelial cells labeled with (I) were treated with singlet oxygen in the presence of 1,4-diazabicyclo[2.2.2]octane, a singlet oxygen quenching agent. The addition of this quenching agent eliminated the ESR spectrum corresponding with (II) and (III). This result is consistent with the quenching of singlet oxygen by 1.4-diazabicyclo[2.2.2]octane. Collectively, our results indicate that extracellularly generated singlet oxygen diffuses into human bronchial epithelial cells and that this process is a potentially important step in the cytotoxic action of singlet oxygen to the respiratory epithelium. |
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Authors:
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A C Nye; G M Rosen; E W Gabrielson; J F Keana; V S Prabhu |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 928 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1987 Apr |
Date Detail:
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Created Date: 1987-05-13 Completed Date: 1987-05-13 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 1-7 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anthracenes
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diagnostic use Bronchi / metabolism* Cells, Cultured Deuterium Diffusion Electron Spin Resonance Spectroscopy Epithelium / metabolism Free Radicals Humans Oxidation-Reduction Oxygen / metabolism* Spin Labels Superoxides / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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GM 27137/GM/NIGMS NIH HHS; HL 33550/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Anthracenes; 0/Free Radicals; 0/Spin Labels; 103438-73-9/N-hydroxytryptacene; 11062-77-4/Superoxides; 7782-39-0/Deuterium; 7782-44-7/Oxygen; 97634-97-4/2-(9,10-dimethoxyanthracenyl)-tert-butylhydroxylamine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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