| Diffusion of rhodamine B and bovine serum albumin in fibrin gels seeded with primary endothelial cells. | |
| | |
MedLine Citation:
|
PMID: 22293601 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Scaffolds with adequate mass transport properties are needed in many tissue engineering applications. Fibrin is considered a good biological material to fabricate such scaffolds. However, very little is known about mass transport in fibrin. Therefore, a method based on the analysis of fluorescence intensity for measuring the apparent diffusion coefficient of rhodamine B and fluorescein-labelled bovine serum albumin (FITC-BSA) is described. The experiments are performed in fibrin gels with and without human umbilical vein endothelial cells (HUVEC). The apparent diffusion coefficients of rhodamine B and FITC-BSA in fibrin (fibrinogen concentration of 4mg/mL) with different cell densities are reported. A LIVE/DEAD(®) assay is performed to confirm the viability of HUVEC seeded at high densities. Diffusion coefficients for rhodamine B remain more or less constant up to 5×10(5)cells/mL and correlate well with literature values measured by other methods in water systems. This indicates that the presence of HUVEC in the fibrin gels (up to 5×10(5)cells/mL) has almost no effect on the diffusion coefficients. Higher cell densities (>5×10(5)cells/mL) result in a decrease of the diffusion coefficients. Diffusion coefficients of rhodamine B and FITC-BSA obtained by this method agree with diffusion coefficients in water predicted by the Stokes-Einstein equation. The experimental design used in this study can be applied to measure diffusion coefficients in different types of gels seeded or not with living cells. |
| | |
Authors:
|
Andriy Shkilnyy; Pierre Proulx; Jamie Sharp; Martin Lepage; Patrick Vermette |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-1-13 |
Journal Detail:
|
Title: Colloids and surfaces. B, Biointerfaces Volume: - ISSN: 1873-4367 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
|
Created Date: 2012-2-1 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9315133 Medline TA: Colloids Surf B Biointerfaces Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2012 Elsevier B.V. All rights reserved. |
Affiliation:
|
Laboratoire de bio-ingénierie et de biophysique de l'Université de Sherbrooke, Department of Chemical and Biotechnological Engineering, Université de Sherbrooke, 2500, boul. de l'Université, Sherbrooke, QC, Canada J1K 2R1. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Role of macrophages in mobilization of hematopoietic progenitor cells from bone marrow after hemorrh...
Next Document: Solid lipid nanoparticles (SLN) - based hydrogels as potential carriers for oral transmucosal delive...