| Diffusion of paramagnetically labeled proteins in cartilage: enhancement of the 1-D NMR imaging technique. | |
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MedLine Citation:
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PMID: 11133285 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Quantifying the diffusive transport of large molecules in avascular cartilage tissue is important both for planning potential pharamacological treatments and for gaining insight into the molecular-scale structure of cartilage. In this work, the diffusion coefficients of gadolinium-DTPA and Gd-labeled versions of four proteins-lysozyme, trypsinogen, ovalbumin, and bovine serum albumin (BSA) with molecular weights of 14,300, 24,000, 45,000, and 67,000, respectively-have been measured in healthy and degraded calf cartilage. The experimental technique relies on the effect of the paramagnetic on the relaxation properties of the surrounding water, combined with the time course of a 1-dimensional spatial profile of the water signal in the cartilage sample. The enhanced technique presented here does not require a prior measurement of the relaxivity of the paramagnetic compound in the sample of interest. The data are expressed as the ratio of the diffusion coefficient of a compound in cartilage to its diffusion coefficient in water. For healthy cartilage, this ratio was 0.34 +/- 0.07 for Gd-DTPA, the smallest compound, and fell to 0.3 +/- 0.1 for Gd-lysozyme, 0.08 +/- 0.04 for Gd-trypsinogen, and 0.07 +/- 0.04 for Gd-ovalbumin. Gd-BSA did not appear to enter healthy cartilage tissue beyond a surface layer. After the cartilage had been degraded by 24-h trypsinization, these ratios were 0.60 +/- 0.03 for Gd-DTPA, 0.40 +/- 0.08 for Gd-lysozyme, 0.42 +/- 0.09 for Gd-trypsinogen, 0.16 +/- 0.14 for Gd-ovalbumin, and 0.11 +/- 0.05 for Gd-BSA. Thus, degradation of the cartilage led to increases in the diffusion coefficient of up to fivefold for the Gd-labeled proteins. These basic transport parameters yield insights on the nature of pore sizes and chemical-matrix interactions in the cartilage tissue and may prove diagnostically useful for identifying the degree and nature of damage to cartilage. |
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Authors:
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B D Foy; J Blake |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of magnetic resonance (San Diego, Calif. : 1997) Volume: 148 ISSN: 1090-7807 ISO Abbreviation: J. Magn. Reson. Publication Date: 2001 Jan |
Date Detail:
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Created Date: 2001-01-26 Completed Date: 2001-06-07 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9707935 Medline TA: J Magn Reson Country: United States |
Other Details:
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Languages: eng Pagination: 126-34 Citation Subset: IM |
Copyright Information:
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Copyright 2001 Academic Press. |
Affiliation:
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Department of Physics, Wright State University, Dayton, Ohio 45435, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Algorithms Animals Cartilage, Articular / chemistry* Cattle Chelating Agents Copper Sulfate Diffusion Electron Spin Resonance Spectroscopy Gadolinium Gels Molecular Weight Muramidase / chemistry Ovalbumin / chemistry Pentetic Acid Proteins / chemistry* Sepharose Serum Albumin, Bovine / chemistry Trypsin Trypsinogen / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Chelating Agents; 0/Gels; 0/Proteins; 0/Serum Albumin, Bovine; 67-43-6/Pentetic Acid; 7440-54-2/Gadolinium; 7758-98-7/Copper Sulfate; 9002-08-8/Trypsinogen; 9006-59-1/Ovalbumin; 9012-36-6/Sepharose; EC 3.2.1.17/Muramidase; EC 3.4.21.4/Trypsin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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