Document Detail

Diffusion measurements in epidermal tissues with fluorescent recovery after photobleaching.
MedLine Citation:
PMID:  18937782     Owner:  NLM     Status:  MEDLINE    
BACKGROUND/PURPOSE: Pressure ulcers are areas of soft tissue breakdown, resulting from sustained mechanical loading of the skin and underlying tissues. Measuring biochemical markers that are released upon mechanical loading by the epidermis seems a promising method for objective risk assessment of the development of pressure ulcers. This risk assessment method will better determine the risk of a patient to develop pressure ulcers than the risk score lists currently used. So far, experimental studies have been performed that measure the tissue response in the culture supernatant. To elucidate the transport of the biochemical markers within the epidermis, the diffusion coefficient needs to be established. METHODS: In the current study, fluorescent recovery after photobleaching (FRAP) is used to determine the diffusion coefficient of fluorescent-labeled dextran molecules in human epidermis, porcine epidermis and engineered epidermal equivalents. These dextran molecules have a similar weight to the biochemical markers. RESULTS: Similar diffusion coefficients were found for human and porcine epidermal samples (6.2 x 10(-5)+/-1.2 x 10(-5) and 5.9 x 10(-5)+/-6.1 x 10(-6) mm2/s, respectively), whereas the diffusion coefficient of the engineered epidermal equivalent was significantly lower (2.3 x 10(-5)+/-1.0 x 10(-5) mm2/s). CONCLUSION: The diffusion could be measured in epidermal tissues using FRAP. In the future, the diffusion coefficients obtained in the current study will be used to study the difference between the transport in EpiDerm cultures and in human epidermis.
Lisette H Cornelissen; Debbie Bronneberg; Cees W J Oomens; Frank P T Baaijens
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)     Volume:  14     ISSN:  1600-0846     ISO Abbreviation:  Skin Res Technol     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-10-21     Completed Date:  2008-12-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9504453     Medline TA:  Skin Res Technol     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  462-7     Citation Subset:  IM    
Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands.
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MeSH Terms
Dextrans / chemistry*,  pharmacokinetics*
Epidermis / physiology*
Fluorescence Recovery After Photobleaching / methods*
Skin / chemistry*
Skin Absorption / physiology*
Reg. No./Substance:

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