| Diffusion and light-dependent compartmentalization of transducin. | |
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MedLine Citation:
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PMID: 21044685 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Diffusion and light-dependent compartmentalization of transducin are essential for phototransduction and light adaptation of rod photoreceptors. Here, transgenic Xenopus laevis models were designed to probe the roles of transducin/rhodopsin interactions and lipid modifications in transducin compartmentalization, membrane mobility, and light-induced translocation. Localization and diffusion of EGFP-fused rod transducin-α subunit (Gα(t1)), mutant Gα(t1) that is predicted to be N-acylated and S-palmitoylated (Gα(t1)A3C), and mutant Gα(t1) uncoupled from light-activated rhodopsin (Gα(t1)-Ctα(s)), were examined by EGFP-fluorescence imaging and fluorescence recovery after photobleaching (FRAP). Similar to Gα(t1), Gα(t1)A3C and Gα(t1)-Ctα(s) were correctly targeted to the rod outer segments in the dark, however the light-dependent translocation of both mutants was markedly impaired. Our analysis revealed a moderate acceleration of the lateral diffusion for the activated Gα(t1) consistent with the diffusion of the separated Gα(t1)GTP and Gβ(1)γ(1) on the membrane surface. Unexpectedly, the kinetics of longitudinal diffusion were comparable for Gα(t1)GTP with a single lipid anchor and heterotrimeric Gα(t1)β(1)γ(1) or Gα(t1)-Ctα(s)β(1)γ(1) with two lipid modifications. This contrasted the lack of the longitudinal diffusion of the Gα(t1)A3C mutant apparently caused by its stable two lipid attachment to the membrane and suggests the existence of a mechanism that facilitates axial diffusion of Gα(t1)β(1)γ(1). |
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Authors:
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Vasily Kerov; Nikolai O Artemyev |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-10-31 |
Journal Detail:
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Title: Molecular and cellular neurosciences Volume: 46 ISSN: 1095-9327 ISO Abbreviation: Mol. Cell. Neurosci. Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-01-10 Completed Date: 2011-07-05 Revised Date: 2012-01-04 |
Medline Journal Info:
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Nlm Unique ID: 9100095 Medline TA: Mol Cell Neurosci Country: United States |
Other Details:
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Languages: eng Pagination: 340-6 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Molecular Physiology and Biophysics, University of Iowa, Iowa City, IA 52242, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Genetically Modified Diffusion Fluorescence Recovery After Photobleaching Light* Protein Subunits / genetics, metabolism Protein Transport Recombinant Fusion Proteins / genetics, metabolism Retinal Rod Photoreceptor Cells / cytology, metabolism* Rhodopsin / metabolism Transducin / genetics, metabolism* Xenopus laevis |
| Grant Support | |
ID/Acronym/Agency:
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R01 EY-12682/EY/NEI NIH HHS; R01 EY012682-12/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Protein Subunits; 0/Recombinant Fusion Proteins; 9009-81-8/Rhodopsin; EC 3.6.1.-/Transducin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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