| Diffusion analysis of glucocorticoid receptor and antagonist effect in living cell nucleus. | |
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MedLine Citation:
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PMID: 17274977 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The diffusion properties of hGRalpha in living cells have been analyzed. The hGRalpha translocalized from the cytosol to the nucleus after addition of Dex just as RU486; however, the Brownian motions of the proteins in nucleus were different. In order to analysis microenvironment of the nucleus of living cell, four different tandem EGFPs were constructed. Diffusion of tandem EGFP was dependent on the length of the protein as a rod-like molecule in solution. We found two kinds of mobility, fast diffusional mobility as a major component and much slower diffusional mobility as a major component in living cells nucleoplasm. On the bases of this analysis, we compared the diffusion property of hGRalpha in the nucleus at the presence of Dex or RU486 by distribution of diffusion constants. Our result may suggest that EGFP-hGRalpha is activated by RU486 and kept the stage of binding cofactor, GRE and final complex. Finally this means that dimerization is not required for association with GRE, although it is required for stabilization of a complex of EGFP-hGRalpha. |
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Authors:
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Shintaro Mikuni; Changi Pack; Mamoru Tamura; Masataka Kinjo |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-12-22 |
Journal Detail:
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Title: Experimental and molecular pathology Volume: 82 ISSN: 0014-4800 ISO Abbreviation: Exp. Mol. Pathol. Publication Date: 2007 Apr |
Date Detail:
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Created Date: 2007-04-02 Completed Date: 2007-05-31 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370711 Medline TA: Exp Mol Pathol Country: United States |
Other Details:
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Languages: eng Pagination: 163-8 Citation Subset: IM |
Affiliation:
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Laboratory of Supramolecular Biophysics, Research Institute for Electronic Science, Hokkaido University, N12W6, Sapporo 060-0812, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Cell Nucleus / drug effects, metabolism* Dexamethasone / pharmacology Diffusion Dimerization Green Fluorescent Proteins / metabolism Hela Cells Humans Mifepristone / pharmacology* Molecular Sequence Data Protein Transport / drug effects Receptors, Glucocorticoid / agonists, antagonists & inhibitors, metabolism* Recombinant Fusion Proteins / agonists, antagonists & inhibitors, metabolism Response Elements |
| Chemical | |
Reg. No./Substance:
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0/Receptors, Glucocorticoid; 0/Recombinant Fusion Proteins; 0/enhanced green fluorescent protein; 0/glucocorticoid receptor alpha; 147336-22-9/Green Fluorescent Proteins; 50-02-2/Dexamethasone; 84371-65-3/Mifepristone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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