| Differentiation of subpopulations of human and murine hemopoietic stem cells by hypotonic lysis. | |
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MedLine Citation:
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PMID: 7356680 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Both human and mouse bone marrow contain subpopulations of hemopoietic stem cells that greatly vary in their resistance to water exposure: The cells forming erythroid colonies or bursts in methyl cellulose in vitro are most sensitive to hypotonic conditions and are destroyed within 60 s in the hypotonic milieu. The murine pluripotent stem cells assayed by the spleen colony technique, as well as both murine and human myeloid stem cells assayed by the plasma clot diffusion chamber technique, displayed intermediate sensitivity and were nearly completely eliminated by 120 s of exposure to water. Both human and mouse bone marrow stem cells producing myeloid colonies in agar are most resistant to hypotonic conditions. The addition of monocyte-macrophages and lymphoid cells to water-exposed mouse bone marrow cell populations to compensate for losses did not restore either erythroid or myeloid colony formation. |
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Authors:
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E Niskanen; M J Cline |
Publication Detail:
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Type: Comparative Study; In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of clinical investigation Volume: 65 ISSN: 0021-9738 ISO Abbreviation: J. Clin. Invest. Publication Date: 1980 Feb |
Date Detail:
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Created Date: 1980-03-24 Completed Date: 1980-03-24 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7802877 Medline TA: J Clin Invest Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 285-9 Citation Subset: AIM; IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Separation / methods Colony-Forming Units Assay Erythropoiesis Female Granulocytes / cytology Hematopoiesis* Hematopoietic Stem Cells / cytology* Humans Mice Osmotic Fragility |
| Comments/Corrections | |
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