| Differentiation of some species of Neisseriaceae and other bacterial groups by DNA-DNA hybridization. | |
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MedLine Citation:
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PMID: 2730785 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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DNA-DNA hybridization using total genomic DNA probes may represent a way of differentiating between miscellaneous bacterial species. This was studied with type and reference strains of 20 species in Moraxella, Kingella, and other selected Gram-negative groups. Both radioactive and biotin labelling were employed. Most of the species examined were easily distinguished, such as Moraxella (Branhamella) catarrhalis, M.(B.) ovis, M. atlantae, M. phenylpyruvica, M. osloensis, Neisseria elongata, N. meningitidis, Kingella kingae, K. indologenes, K. dentrificans, Oligella urethralis, Eikenella corrodens, Cardiobacterium hominis, Haemophilus aphrophilus, Actinobacillus actinomycetemcomitans, Gardnerella vaginalis, and DF-2. This reflected the extent of the genetic distances between them as a basis for identification by hybridization. There was some clustering in the Moraxella group. Especially the closely related Moraxella nonliquefaciens, M. lacunata and M. bovis showed strong hybridization affinities. This leads to potential problems in distinguishing these three species from each other by DNA-DNA hybridization with total genomic probes alone. |
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Authors:
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T Tønjum; G Bukholm; K Bøvre |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica Volume: 97 ISSN: 0903-4641 ISO Abbreviation: APMIS Publication Date: 1989 May |
Date Detail:
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Created Date: 1989-07-25 Completed Date: 1989-07-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8803400 Medline TA: APMIS Country: DENMARK |
Other Details:
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Languages: eng Pagination: 395-405 Citation Subset: IM |
Affiliation:
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Kaptein W. Wilhelmsen og Frues Bakteriologiske Institutt, University of Oslo, Rikshospitalet, Norway. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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DNA Probes DNA, Bacterial / genetics* Moraxella / genetics Neisseriaceae / classification, genetics* Nucleic Acid Hybridization Species Specificity Transformation, Genetic |
| Chemical | |
Reg. No./Substance:
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0/DNA Probes; 0/DNA, Bacterial |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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