Document Detail

Differentiation of some species of Neisseriaceae and other bacterial groups by DNA-DNA hybridization.
MedLine Citation:
PMID:  2730785     Owner:  NLM     Status:  MEDLINE    
DNA-DNA hybridization using total genomic DNA probes may represent a way of differentiating between miscellaneous bacterial species. This was studied with type and reference strains of 20 species in Moraxella, Kingella, and other selected Gram-negative groups. Both radioactive and biotin labelling were employed. Most of the species examined were easily distinguished, such as Moraxella (Branhamella) catarrhalis, M.(B.) ovis, M. atlantae, M. phenylpyruvica, M. osloensis, Neisseria elongata, N. meningitidis, Kingella kingae, K. indologenes, K. dentrificans, Oligella urethralis, Eikenella corrodens, Cardiobacterium hominis, Haemophilus aphrophilus, Actinobacillus actinomycetemcomitans, Gardnerella vaginalis, and DF-2. This reflected the extent of the genetic distances between them as a basis for identification by hybridization. There was some clustering in the Moraxella group. Especially the closely related Moraxella nonliquefaciens, M. lacunata and M. bovis showed strong hybridization affinities. This leads to potential problems in distinguishing these three species from each other by DNA-DNA hybridization with total genomic probes alone.
T Tønjum; G Bukholm; K Bøvre
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  APMIS : acta pathologica, microbiologica, et immunologica Scandinavica     Volume:  97     ISSN:  0903-4641     ISO Abbreviation:  APMIS     Publication Date:  1989 May 
Date Detail:
Created Date:  1989-07-25     Completed Date:  1989-07-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8803400     Medline TA:  APMIS     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  395-405     Citation Subset:  IM    
Kaptein W. Wilhelmsen og Frues Bakteriologiske Institutt, University of Oslo, Rikshospitalet, Norway.
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MeSH Terms
DNA Probes
DNA, Bacterial / genetics*
Moraxella / genetics
Neisseriaceae / classification,  genetics*
Nucleic Acid Hybridization
Species Specificity
Transformation, Genetic
Reg. No./Substance:
0/DNA Probes; 0/DNA, Bacterial

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