Document Detail


Differentiation of renal beta-intercalated cells to alpha-intercalated and principal cells in culture.
MedLine Citation:
PMID:  1608958     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The renal collecting duct is a heterogenous epithelium consisting of intercalated cells (ICC) and principal cells (PC). The origin of this cellular heterogeneity is not clear. To test the hypothesis that the two cell types might originate from one another, pure populations of ICC (beta subtype) and PC were isolated by fluorescence-activated cell sorting and grown on permeable supports. After the monolayers reached confluence, the expression of ICC- and PC-specific functions and antigens was monitored. Cultures of sorted beta-ICC, in addition to expressing ICC-specific functions (such as an electrogenic H+ secretion) and antigens, progressively acquired PC functions (amiloride-sensitive Na+ transport and K+ secretion). On day 6, cultures of sorted beta-ICC exhibited a lumen-negative transepithelial potential difference of 83 +/- 4 mV and a short circuit current of 107 +/- 15 microA/cm2 and created a lumen-to-bath K+ concentration ratio of approximately 10. The percentage of cells staining with two PC-specific antibodies was 53% and 65%. On the other hand, cultures of sorted PC failed to acquire ICC-specific functions while maintaining PC characteristics. To rule out preferential proliferation of a few contaminating PC as an explanation of these results, we have generated a continuous collecting duct cell line (M-1) originating from mice transgenic for the early region of simian virus 40. Cell lines cloned from M-1 cells exhibit both PC and ICC functions and show mutually exclusive heterogenous expression of PC and ICC antigens, demonstrating a common origin of the two cell types. These data indicate that while beta-ICC in culture can give rise to both alpha-ICC and PC, PC cannot convert to ICC, which raises the possibility that beta-ICC is the stem cell in the renal collecting duct. Differentiation of ICC to PC may explain the cellular heterogeneity in the cortical collecting duct.
Authors:
G Fejes-Tóth; A Náray-Fejes-Tóth
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  89     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1992 Jun 
Date Detail:
Created Date:  1992-07-21     Completed Date:  1992-07-21     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  5487-91     Citation Subset:  IM    
Affiliation:
Department of Physiology, Dartmouth Medical School, Hanover, NH 03756.
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MeSH Terms
Descriptor/Qualifier:
Acetazolamide / pharmacology
Animals
Antibodies, Monoclonal
Cell Differentiation
Cell Separation
Cells, Cultured
Clone Cells
Epithelial Cells
Epithelium / physiology
Flow Cytometry / methods
Fluorescein-5-isothiocyanate
Hydrogen-Ion Concentration
Kidney Tubules, Collecting / cytology*,  drug effects,  physiology
Mammals
Membrane Potentials
Potassium / metabolism
Sodium / metabolism
Grant Support
ID/Acronym/Agency:
DK-39523/DK/NIDDK NIH HHS; DK-41841/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 3326-32-7/Fluorescein-5-isothiocyanate; 59-66-5/Acetazolamide; 7440-09-7/Potassium; 7440-23-5/Sodium
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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