| Differentiation-related changes in lipid classes with long-chain and very long-chain polyenoic fatty acids in rat spermatogenic cells. | |
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MedLine Citation:
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PMID: 20610732 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In rat seminiferous tubules (ST), cells that contain polar and neutral lipids with long-chain polyenoic fatty acids (PUFA) and sphingomyelins (SM) and ceramides (Cer) with very long chain (VLC) PUFA of the n-6 series coexist. In this study, pachytene spermatocytes and round spermatids were isolated to determine how these lipids change during spermatogenesis. As the amount per cell of PUFA-rich glycerophospholipids (GPL) decreased with cell size, the 22:5/20:4 ratio increased with cell differentiation. The elovl2 and elovl5 genes, required for 22:5 formation, were expressed (mRNA) in both cell types. Residual bodies- particles with compacted organelles and materials discarded from late spermatids-concentrated cholesterol, 22:5-rich triacylglycerols, and GPL, including plasmalogens and phosphatidylserine. Species of SM and Cer with nonhydroxylated (n-) VLCPUFA (28:4, 30:5, and 32:5) predominated in pachytene spermatocytes, whereas species with the corresponding 2-hydroxy (2-OH) VLCPUFA prevailed in round spermatids. Thus, a dramatic increase in the 2-OH/n-VLCPUFA ratio in SM and Cer was a hallmark of differentiation. A substantial decrease of 2-OH SM occurred between spermatids and mature spermatozoa and 2-OH SM species were collected in residual bodies "en route" to Sertoli cells. Notably, spermatids and spermatozoa gained a significant amount of ceramides devoid of n-VLCPUFA but having 2-OH VLCPUFA as their main fatty acids. |
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Authors:
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Gerardo M Oresti; Juan G Reyes; Jessica M Luquez; Nelson Osses; Natalia E Furland; Marta I Aveldaño |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-07-07 |
Journal Detail:
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Title: Journal of lipid research Volume: 51 ISSN: 0022-2275 ISO Abbreviation: J. Lipid Res. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-10 Completed Date: 2011-01-24 Revised Date: 2013-05-29 |
Medline Journal Info:
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Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: United States |
Other Details:
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Languages: eng Pagination: 2909-21 Citation Subset: IM |
Affiliation:
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Instituto de Investigaciones Bioquímicas de Bahía Blanca, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) y Universidad Nacional del Sur (UNS), Bahía Blanca, Argentina. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetyltransferases
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genetics,
metabolism Animals Cell Differentiation* Ceramides / metabolism Fatty Acids, Unsaturated / metabolism* Male Rats Rats, Wistar Spermatids / chemistry*, cytology, metabolism* Spermatocytes / cytology, metabolism* Spermatogenesis Sphingomyelins / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Ceramides; 0/Fatty Acids, Unsaturated; 0/Sphingomyelins; EC 2.3.1.-/Acetyltransferases; EC 2.3.1.-/fatty acid elongases |
| Comments/Corrections | |
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