Document Detail


Differentiation-related changes in lipid classes with long-chain and very long-chain polyenoic fatty acids in rat spermatogenic cells.
MedLine Citation:
PMID:  20610732     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In rat seminiferous tubules (ST), cells that contain polar and neutral lipids with long-chain polyenoic fatty acids (PUFA) and sphingomyelins (SM) and ceramides (Cer) with very long chain (VLC) PUFA of the n-6 series coexist. In this study, pachytene spermatocytes and round spermatids were isolated to determine how these lipids change during spermatogenesis. As the amount per cell of PUFA-rich glycerophospholipids (GPL) decreased with cell size, the 22:5/20:4 ratio increased with cell differentiation. The elovl2 and elovl5 genes, required for 22:5 formation, were expressed (mRNA) in both cell types. Residual bodies- particles with compacted organelles and materials discarded from late spermatids-concentrated cholesterol, 22:5-rich triacylglycerols, and GPL, including plasmalogens and phosphatidylserine. Species of SM and Cer with nonhydroxylated (n-) VLCPUFA (28:4, 30:5, and 32:5) predominated in pachytene spermatocytes, whereas species with the corresponding 2-hydroxy (2-OH) VLCPUFA prevailed in round spermatids. Thus, a dramatic increase in the 2-OH/n-VLCPUFA ratio in SM and Cer was a hallmark of differentiation. A substantial decrease of 2-OH SM occurred between spermatids and mature spermatozoa and 2-OH SM species were collected in residual bodies "en route" to Sertoli cells. Notably, spermatids and spermatozoa gained a significant amount of ceramides devoid of n-VLCPUFA but having 2-OH VLCPUFA as their main fatty acids.
Authors:
Gerardo M Oresti; Juan G Reyes; Jessica M Luquez; Nelson Osses; Natalia E Furland; Marta I Aveldaño
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-07-07
Journal Detail:
Title:  Journal of lipid research     Volume:  51     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-10     Completed Date:  2011-01-24     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2909-21     Citation Subset:  IM    
Affiliation:
Instituto de Investigaciones Bioquímicas de Bahía Blanca, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) y Universidad Nacional del Sur (UNS), Bahía Blanca, Argentina.
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MeSH Terms
Descriptor/Qualifier:
Acetyltransferases / genetics,  metabolism
Animals
Cell Differentiation*
Ceramides / metabolism
Fatty Acids, Unsaturated / metabolism*
Male
Rats
Rats, Wistar
Spermatids / chemistry*,  cytology,  metabolism*
Spermatocytes / cytology,  metabolism*
Spermatogenesis
Sphingomyelins / metabolism
Chemical
Reg. No./Substance:
0/Ceramides; 0/Fatty Acids, Unsaturated; 0/Sphingomyelins; EC 2.3.1.-/Acetyltransferases; EC 2.3.1.-/fatty acid elongases
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