Document Detail

Differentiation of rat pheochromocytoma cells by fomitellic acids, specific DNA polymerase inhibitors.
MedLine Citation:
PMID:  10983859     Owner:  NLM     Status:  MEDLINE    
Fomitellic acid (FA) A and B are specific inhibitors of DNA polymerase alpha and beta. They showed cytotoxicity against rat pheochromocytoma cells (PC-12 cells) in a concentration-dependent manner. However, after PC-12 cells were cultivated with low concentrations of FAs, the cells extended neurites in greater degree similar to the cells cultivated with nerve growth factor. Another DNA polymerase alpha inhibitor, aphidicolin, also induced neurite outgrowth. Furthermore, PC-12 cells were strongly immunostained with anti-alpha-tubulin or anti-tau antibody after the treatment with FAs. These results suggest that weak inhibition of DNA polymerase activity induces the neurite outgrowth in PC-12 cells.
Y Obara; N Nakahata; Y Mizushina; F Sugawara; K Sakaguchi; Y Ohizumi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Life sciences     Volume:  67     ISSN:  0024-3205     ISO Abbreviation:  Life Sci.     Publication Date:  2000 Aug 
Date Detail:
Created Date:  2000-09-22     Completed Date:  2000-09-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375521     Medline TA:  Life Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1659-65     Citation Subset:  IM    
Department of Pharmaceutical Molecular Biology, Graduate School of Pharmaceutical Sciences, Tohoku University, Japan.
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MeSH Terms
Cell Differentiation / drug effects*
DNA Polymerase I / antagonists & inhibitors
DNA Polymerase beta / antagonists & inhibitors
Enzyme Inhibitors / pharmacology*,  toxicity
Growth Cones / drug effects
Inhibitory Concentration 50
Neurites / drug effects
PC12 Cells
Terpenes / pharmacology*,  toxicity
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Terpenes; 0/fomitellic acid A; 0/fomitellic acid B; EC 2.7.7.-/DNA Polymerase I; EC 2.7.7.-/DNA Polymerase beta

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