| Differentiation of probiotic and environmental Saccharomyces cerevisiae strains in animal feed. | |
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MedLine Citation:
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PMID: 20337759 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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AIMS: To establish an identification system for probiotic Saccharomyces cerevisiae strains based on artificial neural network (ANN)-assisted Fourier-transform infrared (FTIR) spectroscopy to improve quality control of animal feed. METHODS AND RESULTS: The ANN-based system for differentiating environmental from probiotic S. cerevisiae strains comprises five authorized feed additive strains plus environmental strains isolated from different habitats. A total of 108 isolates were used as reference strains to create the ANN. DHPLC analysis and δ-PCR were used as reference methods to type probiotic yeast isolates. The performance of the FTIR-ANN was tested in an internal validation using unknown spectra of each reference strain. This validation step yielded a classification rate of 99·1 %. For an external validation, a test data set comprising 965 spectra of 63 probiotic and environmental S. cerevisiae isolates unknown to the ANN was used, resulting in a classification rate of 98·2 %. CONCLUSIONS: Our results demonstrate that probiotic S. cerevisiae strains in feed can be differentiated successfully from environmental isolates using both genotypic approaches and ANN-based FTIR spectroscopy. SIGNIFICANCE AND IMPACT OF THE STUDY: FTIR-based artificial neural network analysis provides a rapid and inexpensive technique for yeast identification both at the species and at the strain level in routine diagnostic laboratories, using a single sample preparation. |
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Authors:
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N R Büchl; M Hutzler; H Mietke-Hofmann; M Wenning; S Scherer |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of applied microbiology Volume: 109 ISSN: 1365-2672 ISO Abbreviation: J. Appl. Microbiol. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-11-05 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9706280 Medline TA: J Appl Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 783-91 Citation Subset: IM |
Copyright Information:
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© 2010 The Authors. Journal compilation © 2010 The Society for Applied Microbiology. |
Affiliation:
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Abteilung Mikrobiologie, Zentralinstitut für Ernährungs- und Lebensmittelforschung (ZIEL), Technische Universität München, Freising, Germany. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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