Document Detail


Differentiation of pathogenic Entamoeba histolytica from other intestinal protozoa by riboprinting.
MedLine Citation:
PMID:  1364099     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Differentiation of the pathogen Entamoeba histolytica from the variety of other amebas that can infect the human intestinal tract is vital for accurate diagnosis and treatment. Morphology and serology alone are not adequate for positive identification to be achieved. We have developed methods using the polymerase chain reaction to amplify amebal ribosomal RNA genes that allow either specific detection of E. histolytica or species identification.
Authors:
C G Clark; L S Diamond
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Archives of medical research     Volume:  23     ISSN:  0188-4409     ISO Abbreviation:  Arch. Med. Res.     Publication Date:  1992  
Date Detail:
Created Date:  1993-12-20     Completed Date:  1993-12-20     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9312706     Medline TA:  Arch Med Res     Country:  MEXICO    
Other Details:
Languages:  eng     Pagination:  15-6     Citation Subset:  IM    
Affiliation:
Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
DNA, Protozoan / analysis*
DNA, Ribosomal / analysis*
Entamoeba / classification
Entamoeba histolytica / genetics,  isolation & purification*
Eukaryota / genetics*
Molecular Sequence Data
Polymerase Chain Reaction*
Polymorphism, Restriction Fragment Length
RNA, Protozoan / genetics
RNA, Ribosomal / genetics
Species Specificity
Chemical
Reg. No./Substance:
0/DNA, Protozoan; 0/DNA, Ribosomal; 0/RNA, Protozoan; 0/RNA, Ribosomal

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