Document Detail


Differentiation of organic goat's milk based on its hippuric acid content as determined by capillary electrophoresis.
MedLine Citation:
PMID:  20593395     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Organic foods have lately aroused interest by virtue of their quality and their essential, exclusive characteristics. In this study, we assessed the potential of CE to detect a marker that is able to identify the particular food given to the goats and develop an effective method to determine directly the most abundant organic acid in goat's milk (viz. hippuric acid). In addition, we examined the use of the hippuric acid content of goat's milk as a general marker for authenticating organic goat's milk. The feeding of these goats can be influenced by the time of the year. For this reason, we have collected samples from 20 conventionally fed goats and 20 organically fed goats over a period of 2 months. A threshold value dependent on the hippuric acid content was thus established for the first time with a view to discriminating between conventional and organic goat's milk. Organic acids in goat's milk were separated in a running buffer consisting of 120 mM sodium tetraborate decahydrate and 0.5 mM CTAB set to pH 8. The precision of the ensuing method is acceptable for hippuric acid; thus, the RSD for peak area and migration time was less than 10 and 4%, respectively. Also, calibration curves were linear throughout the studied concentration range.
Authors:
Azahara Carpio; Vicente Rodríguez-Estévez; Manuel Sánchez-Rodríguez; Lourdes Arce; Miguel Valcárcel
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Electrophoresis     Volume:  31     ISSN:  1522-2683     ISO Abbreviation:  Electrophoresis     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-07-05     Completed Date:  2010-09-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  2211-7     Citation Subset:  IM    
Affiliation:
Department of Analytical Chemistry, University of Córdoba, Córdoba, Spain.
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MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Animal Feed
Animals
Carboxylic Acids
Cetrimonium Compounds
Electrophoresis, Capillary / methods*
Food Analysis / methods*
Goats
Hippurates / analysis*
Linear Models
Milk / chemistry*
Principal Component Analysis
Reproducibility of Results
Statistics, Nonparametric
Chemical
Reg. No./Substance:
0/Carboxylic Acids; 0/Cetrimonium Compounds; 0/Hippurates; 495-69-2/hippuric acid; 6899-10-1/cetrimonium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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