Document Detail

Differentiation of murine premigratory primordial germ cells in culture.
MedLine Citation:
PMID:  10491656     Owner:  NLM     Status:  MEDLINE    
In the mouse embryo, primordial germ cells first appear in the extraembryonic mesoderm and divide rapidly while migrating to the fetal gonad. Shortly after their arrival in the gonad, germ cells sexually differentiate as proliferation ceases. Previous studies have established that primordial germ cells proliferate and migrate in feeder layer culture. To explore cellular regulation of fetal germ cell development, we have used germ cell nuclear antigen 1 (GCNA1), a marker normally expressed only in postmigratory germ cells, to investigate the developmental potency of both pre- and postmigratory cells in this culture system. We found that explanted premigratory germ cells will initiate expression of this marker and are, therefore, capable of undertaking some aspects of gonocyte differentiation without intimate exposure to the fetal gonad. We have also tested whether postmigratory gonocytes are stable in culture. As detected by either alkaline phosphatase or GCNA1, we did not detect long-term survival of either prospermatogonia or oogonia under conditions that support the survival, proliferation, and differentiation of earlier premigratory cells. These observations are consistent with an autonomous cellular mechanism governing the initial stages of gonocyte differentiation, and suggest that differentiation towards gonocytes is accompanied by a change in requirements for cell survival.
A J Richards; G C Enders; J L Resnick
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Biology of reproduction     Volume:  61     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  1999 Oct 
Date Detail:
Created Date:  1999-11-22     Completed Date:  1999-11-22     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1146-51     Citation Subset:  IM    
Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, Florida 32610-0266, USA.
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MeSH Terms
Alkaline Phosphatase / metabolism
Cell Differentiation*
Cell Movement
Cells, Cultured
DNA-Binding Proteins / biosynthesis
Germ Cells / cytology*
Nuclear Receptor Subfamily 6, Group A, Member 1
Receptors, Cytoplasmic and Nuclear / biosynthesis
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Nr6a1 protein, mouse; 0/Nuclear Receptor Subfamily 6, Group A, Member 1; 0/Receptors, Cytoplasmic and Nuclear; EC Phosphatase

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