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Differentiation of mammary stem cells in vivo and in vitro.
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MedLine Citation:
PMID:  2647483     Owner:  NLM     Status:  MEDLINE    
The fully differentiated cells of the rat mammary parenchyma, the ductal epithelial, alveolar, and myoepithelial cells, are distinguished by their ultrastructure and by their accumulation of immunocytochemically detectable marker proteins. The different cell types probably develop from primative ductal structures called terminal end buds, which are present in the developing rat mammary glands, and these structures contain relatively undifferentiated cells. Clonal epithelial stem cell lines, obtained from normal rat mammary glands or benign mammary tumors, differentiate under appropriate conditions along a pathway to droplet-cell/doming cultures of primative alveolarlike cells. Under different culture conditions, the epithelial stem cells differentiate along a separate pathway to myoepitheliallike cells. They accumulate some of the specific marker proteins of myoepithelial cells in vivo, including type IV collagen, laminin, and Thy-1 antigen. In addition, these myoepitheliallike cells in culture contain an abundance of a potential calcium-binding protein, p9Ka, which also occurs in myoepithelial cells of histological sections from mammary glands. The accumulation of type IV collagen, laminin, Thy-1, and p9Ka occurs asynchronously along the pathway to the myoepitheliallike cells in vitro. Furthermore, the steady-state levels of these different marker proteins arise by alterations in the controls at the transcriptional, the posttranscriptional processing, and the translational stages of their production. These results suggest a stepwise control of synthesis of myoepithelial cell marker proteins, and in the case of p9Ka and Thy-1 antigen, this altered control may arise through their possession of novel transcriptional promoters.
R Barraclough; P S Rudland
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Environmental health perspectives     Volume:  80     ISSN:  0091-6765     ISO Abbreviation:  Environ. Health Perspect.     Publication Date:  1989 Mar 
Date Detail:
Created Date:  1989-05-10     Completed Date:  1989-05-10     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0330411     Medline TA:  Environ Health Perspect     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  39-48     Citation Subset:  IM    
Department of Biochemistry, University of Liverpool, UK.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Calcium-Binding Proteins / genetics
Cell Differentiation*
Mammary Glands, Animal / cytology*
Mammary Neoplasms, Experimental / pathology
Molecular Sequence Data
Neoplastic Stem Cells / cytology
Stem Cells / cytology*
Reg. No./Substance:
0/Calcium-Binding Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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Journal Information
Journal ID (nlm-ta): Environ Health Perspect
ISSN: 0091-6765
Article Information
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Print publication date: Month: 3 Year: 1989
Volume: 80First Page: 39 Last Page: 48
ID: 1567605
PubMed Id: 2647483

Differentiation of mammary stem cells in vivo and in vitro.
R Barraclough
P S Rudland
Department of Biochemistry, University of Liverpool, UK.

Article Categories:
  • Research Article

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