Document Detail


Differentiation-induced and constitutive transcription of human papillomavirus type 31b in cell lines containing viral episomes.
MedLine Citation:
PMID:  1326657     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The expression of viral genes during the productive life cycle of human papillomaviruses (HPV) is tightly coupled to the differentiation program of epithelial cells. We have examined transcription of HPV as a function of differentiation in an in vitro organotypic raft culture system which allows for epithelial stratification at the air-liquid interface. When CIN612 cells, which contain episomal copies of HPV type 31b (HPV31b), were allowed to stratify in raft cultures, they differentiated in a manner which was histologically similar to that seen in a cervical intraepithelial neoplasia I biopsy lesion. In monolayer cultures of CIN612 cells, two major polycistronic HPV31b transcripts of 1.7 kb which encode (i) E6, E7, E1-E4, and E5 and (ii) E6*, E7, E1-E4, and E5 were identified. These RNAs initiated at a promoter, P97, in the upstream regulatory region of the virus. Following differentiation in raft cultures, the relative abundance of RNAs initiated at P97 was unchanged. In contrast, the expression of a 1.3-kb RNA encoding an E1-E4 fusion protein and E5 was found to increase substantially following differentiation. This transcript was initiated at a novel promoter within the E7 gene (P742). These studies have therefore identified a constitutive viral promoter which is active throughout stratified epithelium as well as a novel promoter which is induced upon epithelial cell differentiation.
Authors:
M Hummel; J B Hudson; L A Laimins
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of virology     Volume:  66     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1992 Oct 
Date Detail:
Created Date:  1992-10-19     Completed Date:  1992-10-19     Revised Date:  2010-09-07    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  6070-80     Citation Subset:  IM    
Affiliation:
Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Base Sequence
Blotting, Northern
Blotting, Southern
Cell Differentiation*
Cell Line
Cloning, Molecular
DNA, Viral
Genes, Viral
Humans
Keratinocytes / metabolism
Molecular Sequence Data
Open Reading Frames
Papillomaviridae / genetics*
Plasmids*
Transcription, Genetic*
Chemical
Reg. No./Substance:
0/DNA, Viral
Comments/Corrections

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