| Differentiation of individual human mesenchymal stem cells probed by FTIR microscopic imaging. | |
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MedLine Citation:
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PMID: 17592583 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Objective of this study is the novel application of Fourier transform infrared (FTIR) microscopic imaging to identify the differentiation state of individual human mesenchymal stem cells with or without osteogenic stimulation. IR spectra of several hundred single cells with lateral resolution of 5-10 microm were recorded using a FTIR imaging spectrometer coupled to a microscope with a focal plane array detector. A classification model based on linear discriminant analysis was trained to distinguish four cell types by their IR spectroscopic fingerprint. Without stimulation two cell types dominated, showing low or high levels of glycogen accumulation at the cell periphery. After stimulation, the protein composition in the cells changed and some cells started expressing calcium phosphate salts such as octacalciumphosphate, a precursor of the bone constituent hydroxyapatite. Few cells were identified which remained in their non-stimulated state. This study demonstrated for the first time that FTIR microscopic imaging can probe stem cell differentiation at the single cell level rapidly, non-destructively and with minimal preparation. |
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Authors:
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Christoph Krafft; Reiner Salzer; Sebastian Seitz; Christina Ern; Matthias Schieker |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-05-24 |
Journal Detail:
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Title: The Analyst Volume: 132 ISSN: 0003-2654 ISO Abbreviation: Analyst Publication Date: 2007 Jul |
Date Detail:
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Created Date: 2007-06-26 Completed Date: 2007-11-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372652 Medline TA: Analyst Country: England |
Other Details:
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Languages: eng Pagination: 647-53 Citation Subset: IM |
Affiliation:
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Institute for Analytical Chemistry, Dresden University of Technology, D-01062 Dresden, Germany. christoph.krafft@tu-dresden.de |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Brain Neoplasms
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diagnosis* Cell Differentiation Discriminant Analysis Humans Mesenchymal Stem Cells / pathology*, ultrastructure Spectroscopy, Fourier Transform Infrared / methods |
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