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Differentiation of hematopoietic stem cell and myeloid populations by ATP is modulated by cytokines.
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PMID:  21633388     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Extracellular nucleotides are emerging as important regulators of inflammation, cell proliferation and differentiation in a variety of tissues, including the hematopoietic system. In this study, the role of ATP was investigated during murine hematopoiesis. ATP was able to reduce the percentage of hematopoietic stem cells (HSCs), common myeloid progenitors and granulocyte-macrophage progenitors (GMPs), whereas differentiation into megakaryocyte-erythroid progenitors was not affected. In addition, in vivo administration of ATP to mice reduced the number of GMPs, but increased the number of Gr-1(+)Mac-1(+) myeloid cells. ATP also induced an increased proliferation rate and reduced Notch expression in HSCs and impaired HSC-mediated bone marrow reconstitution in sublethally irradiated mice. Moreover, the effects elicited by ATP were inhibited by suramin, a P2 receptor antagonist, and BAPTA, an intracellular Ca(2+) chelator. We further investigated whether the presence of cytokines might modulate the observed ATP-induced differentiation. Treatment of cells with cytokines (stem cell factor, interleukin-3 and granulocyte-monocyte colony stimulator factor) before ATP stimulation led to reduced ATP-dependent differentiation in long-term bone marrow cultures, thereby restoring the ability of HSCs to reconstitute hematopoiesis. Thus, our data suggest that ATP induces the differentiation of murine HSCs into the myeloid lineage and that this effect can be modulated by cytokines.
Authors:
C M V Barbosa; C M M P Leon; A Nogueira-Pedro; F Wasinsk; R C Araújo; A Miranda; A T Ferreira; E J Paredes-Gamero
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-06-02
Journal Detail:
Title:  Cell death & disease     Volume:  2     ISSN:  2041-4889     ISO Abbreviation:  Cell Death Dis     Publication Date:  2011  
Date Detail:
Created Date:  2011-06-02     Completed Date:  2012-03-01     Revised Date:  2013-06-28    
Medline Journal Info:
Nlm Unique ID:  101524092     Medline TA:  Cell Death Dis     Country:  England    
Other Details:
Languages:  eng     Pagination:  e165     Citation Subset:  IM    
Affiliation:
Departamento de Biofísica, Universidade Federal de São Paulo, Rua Botucatu 862, 2° Andar, 04023-062 São Paulo, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / metabolism*
Animals
Calcium / analysis,  metabolism
Cell Differentiation*
Cell Proliferation
Cytokines / metabolism*
Hematopoietic Stem Cells / cytology*,  metabolism
Mice
Mice, Inbred C57BL
Myeloid Progenitor Cells / cytology*,  metabolism
Chemical
Reg. No./Substance:
0/Cytokines; 56-65-5/Adenosine Triphosphate; 7440-70-2/Calcium
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Journal Information
Journal ID (nlm-ta): Cell Death Dis
ISSN: 2041-4889
Publisher: Nature Publishing Group
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Copyright © 2011 Macmillan Publishers Limited
open-access:
Received Day: 24 Month: 01 Year: 2011
Revision Received Day: 05 Month: 04 Year: 2011
Accepted Day: 28 Month: 04 Year: 2011
Print publication date: Month: 06 Year: 2011
Electronic publication date: Day: 02 Month: 06 Year: 2011
pmc-release publication date: Day: 1 Month: 6 Year: 2011
Volume: 2 Issue: 6
First Page: e165 Last Page:
ID: 3168991
Publisher Item Identifier: cddis201149
DOI: 10.1038/cddis.2011.49
PubMed Id: 21633388

Differentiation of hematopoietic stem cell and myeloid populations by ATP is modulated by cytokines Alternate Title:Myeloid differentiation by ATP
C M V Barbosa1
C M M P Leon1
A Nogueira-Pedro1
F Wasinsk1
R C Araújo1
A Miranda1
A T Ferreira1
E J Paredes-Gamero12*
1Departamento de Biofísica, Universidade Federal de São Paulo, Rua Botucatu 862, 2° Andar, 04023-062 São Paulo, Brazil
2Departamento de Bioquímica, Universidade Federal de São Paulo, Rua Três de Maio 100, 04044-020 São Paulo, Brazil
*Departamento de Biofísica, Universidade Federal de São Paulo, Rua Botucatu 862, 2° Andar, 04023-062, São Paulo, Brazil. Tel: +55 115 572 4583; Fax: +55 115 571 5780; E-mail: edgar.gamero@unifesp.br

Under steady-state conditions, hematopoietic bone marrow cells develop in intimate association with a highly organized, three-dimensional microenvironment. This structural scaffold is composed of many cell types, including osteoblasts and stromal cells, as well as their associated biosynthetic products: components of the extracellular matrix, adhesion molecules and hematopoietic growth factors.1 The specific bone marrow microenvironment in which hematopoietic stem cells (HSCs) reside is referred to as the stem cell niche. HSCs are a subset of bone marrow cells that are capable of self-renewal and of forming all types of blood cells.2 The hematopoietic niche has an essential role in regulating the self-renewal and differentiation of stem cells. In the bone marrow, two stem niches have been characterized: the osteoblastic niche, where osteoblasts primarily control the quiescence and asymmetric proliferation of HSCs,3 and the vascular niche, which is mainly associated with myeloid differentiation.4

The cells present in hematopoietic niches regulate hematopoiesis through the release of different cytokines, which then activate distinct cytokine receptors with intrinsic kinase activity or Janus kinase-dependent receptors. However, new families of G-protein-coupled receptors and ion channels have been recently associated with the regulation of hematopoiesis,5 such as the P2 receptor family, which is activated by extracellular nucleotides.6, 7 These extracellular nucleotides are released by endothelial and osteoblast cells,8, 9, 10 and may be associated with myeloid differentiation.6

The functions of ATP are mediated by receptors of the P2 receptor family, which are further divided into the P2X (ionotropic receptor) and P2Y (metabotropic receptor) subfamilies.11 P2 receptor activation triggers many physiological responses in various cell types that are mediated by a Ca2+ influx through the P2X receptor or via intracellular Ca2+ (Ca2+i) release by P2Y receptors that activate phospholipase C (PLC). PLC hydrolyzes phosphatidylinositol-4,5-bisphosphate and a subsequent increase in inositol 1,4,5-trisphosphate (InsP3) and diacylglycerol (DAG) occurs.12 In addition, changes in the level of adenylate cyclase activity have been shown to influence the concentration of cyclic adenosine monophosphate.13, 14 The activation of P2 receptors controls other intracellular pathways by changing the concentration levels of Ca2+i and cyclic adenosine monophosphate, modulating the mitogen-activated protein kinases and phosphoinositide 3-kinase, both related with proliferation and differentiation.15, 16

In hematopoietic cells, treatment with ATP leads to cellular activation, with an increase in proinflammatory activities, cell death,17, 18 platelet aggregation,14 and cellular proliferation and differentiation.6, 7 Studies in promyelocytic leukemia HL-60 cells have shown that the expression levels of P2 receptors change significantly during myeloid differentiation.19, 20 Nucleotides such as ATP and UTP enhance the response of HSCs to several cytokines.7 For example, UTP was able to significantly improve chemokine receptor 4-mediated (CxC4R) HSC migration.21 In addition, we have shown that large increases in ATP-induced Ca2+i promote transient proliferation and induce the differentiation of primitive hematopoietic cells in long-term bone marrow cultures (LTBMCs).6 Recently, the functional presence of P2X receptors was described in an HSC subset, which is probably related to proliferation.22

To understand the physiological role of P2 receptors in the hematopoietic system, the capacity of extracellular ATP to promote differentiation was investigated in the bone marrow. Furthermore, the ability of cytokines to inhibit ATP-dependent differentiation was explored. Our data show that extracellular ATP induces a reduction in the number of HSCs and myeloid progenitors (MPs), which leads to an increase of mature myeloid cells in the bone marrow, and that this effect can be modulated by the presence of cytokines.


Results
ATP induces changes in the percentage of primitive and mature bone marrow populations

Because ATP induces the differentiation of primitive cells in LTBMCs, we determined whether ATP stimulation could alter the percentages of the HSC and MP populations, including the common myeloid progenitors (CMPs), granulocyte–macrophage progenitors (GMPs) and megakaryocytic–erythroid progenitors (MEPs). An HSC phenotype was defined in this study as Linc-Kit+Sca-1+Thy1.1+Flk2, a subset capable of extensive self-renewal and differentiation into all types of blood cells.23 Other myeloid progenitors were recognized as described in previous reports.2, 23 The identification of the populations used in this study is provided in the Supplementary information (Supplementary Figure 1). For this purpose, bone marrow cells were stimulated with ATP at different times and concentrations. Following stimulation, the samples were labeled to permit the identification of primitive hematopoietic populations by flow cytometry. A high concentration of ATP (1 mM) was required to promote different effects in the hematopoietic cells.7, 24 However, this concentration did not induce hematopoietic cell death6, 17 (Supplementary Figure 2). Concentrations lower than 1 mM did not induce significant changes in the percentage of HSCs (Supplementary Figure 3).

Our results showed a significant decrease in the percentage of the HSC (to 44% of untreated levels), CMP (to 28% of untreated levels) and GMP (to 27% of untreated levels) populations after stimulation with ATP for 4 h in vitro (Figure 1a), whereas the MEP population was not affected. Similar results were obtained by stimulation performed in short culture for 12 h (Supplementary Figure 4A).

To verify the ATP-induced differentiation, a dose of 0.47 g/kg of ATP was administered in vivo for 4 days. This dose injected intraperitoneally has been used previously in other studies to stimulate P2 receptors and did not induce adverse effects in the treated animals.25, 26 After treatment with ATP, bone marrow cells were collected for immunophenotyping. An evaluation of the hematopoietic cell populations showed a significant reduction in GMPs (40%). The HSC population was reduced in 35% (of untreated levels), although this did not constitute a significant decrease, whereas the CMP and MEP populations were not affected (Figure 1b). Furthermore, an evaluation of the mature myeloid population (Gr-1+Mac-1+) from experiments performed in vivo indicated a significant increase (43%) after 4 days of treatment (Figure 1c).

In additionally, suramin, a P2 antagonist were used to verify the participation of P2 receptors in the observed effects. Suramin was able to inhibit the decrease of the hematopoietic cell populations induced by ATP, both in vivo and in vitro (Figure 1a and b). Moreover, in the absence of ATP, suramin did not affect the percentage of hematopoietic populations (Supplementary Figures 4B and C). Furthermore, adenosine, which is produced by ATP degradation activating P1 receptors, and xanthine, an adenosine receptor antagonist, were used to evaluate P1 receptors' involvement. From our results, we observed that adenosine did not reduce the percentage of hematopoietic populations (Supplementary Figures 5A and B) and that xanthine did not abolish the effect of ATP in vivo and in vitro (Supplementary Figures 5C and D).

Furthermore, the number of colony-forming units of granulocytes and macrocytes (CFU-GM) and burst-forming units-erythroid (BFU-E) was quantified using methylcellulose assays. ATP induced a 42% reduction in the number of CFU-GM, but did not alter the number of BFU-E (Figure 1d). Concentrations lower than 1 mM did not induce significant changes in the percentage of CFU-GM colonies (Supplementary Figure 6). These data strongly suggest that ATP is involved in myeloid differentiation, reducing primitive MPs and increasing their mature forms.

Moreover, as the presence of macrophages in whole bone marrow could participate indirectly in the effects of differentiation induced by ATP, the same test was conducted with isolated c-Kit+ cells cultured on the stroma cell line MS5 for 24 h. Similar effects of differentiation in response to ATP were observed in the absence of macrophages (Figure 1e).

ATP reduces HSC potency

Subsequently, other tests were performed to verify the ability of ATP to promote myeloid differentiation, and important features of HSCs were evaluated, such as exhibiting a quiescent state, Notch receptor expression and the ability of HSCs to repopulate the bone marrow. We measure the proliferative state using an antibody against Ki-67 protein, which is specifically expressed during all active phases of the cell cycle, but is absent from resting cells. Stimulation of bone marrow cells with ATP induced an increase of the HSC-Ki-67+ population from 20±1 to 46±3%, showing that ATP induces HSC proliferation (Figure 2a). Notch receptors have been associated with the self-renewal, quiescence, maintenance and differentiation of HSC, and its expression is used as an indicator of the undifferentiated state of HSC.27, 28 Stimulation of bone marrow cells with 1 mM ATP for 2 or 4 h led to a marked reduction in the Notch receptor expression of the HSC and MP populations (Figure 2b). In addition, an evaluation of the competence of HSCs to repopulate the bone marrow microenvironment after stimulation with 1 mM ATP was performed using transgenic mice expressing the green fluorescent protein (GFP). Freshly isolated bone marrow cells from GFP animals were stimulated with ATP for 4 h and immediately injected by retro-orbital injection into sublethally irradiated (700 Gy) wild-type animals. After 8 weeks, the animals were killed, and the frequency of GFP+ cells within the different hematopoietic populations was determined. The hematopoietic populations from the ATP-stimulated group showed a clear reduction in the frequency of GFP+ cells compared with controls, indicating a decrease in the ability of ATP-treated HSCs to reconstitute the hematopoietic system (Figure 2c). Furthermore, the expression of CxCR4 and calcium-sensing receptors (CaSR), which are homing receptors of HSCs, were evaluated. Stimulation of bone marrow cells in vitro did not alter CxCR4 or CaSR expression (Supplementary Figures 7A and B).

Osteoblastic and endothelial niches regulate the quiescent state of HSCs. Therefore, if ATP induces HSC differentiation, then it will likely result in a high level of expression of ectonucleotidases in hematopoietic progenitors (HPs) and osteoblast cells. The expression of CD39 and CD73, which hydrolyze ATP into ADP and AMP into adenosine, respectively, was analyzed in freshly isolated bone marrow cells. HSC, HP and stromal cells express CD39 and CD73 (Supplementary Figures 7C–F). Moreover, activity of ectonucleotidases was compared among three populations: immature hematopoietic population (c-Kit+ cells), stromal cells (CD45Ter-119) and mature cells of bone marrow (whole bone marrow cells). We observed that immature hematopoietic cells exhibit a higher ectonucleotidase activity than mature or stromal cells (Supplementary Figure 8).

ATP promotes increased [Ca2+]i in hematopoietic cells

The observed ATP-induced differentiation in LTBMCs is related to a large increase in Ca2+i.6 To verify that the differentiation of primitive bone marrow hematopoietic cells is dependent on Ca2+i signaling, initial measurements of Ca2+i were carried out in freshly isolated HSC and c-Kit+Lin populations (a population that contains HPs). After separation, the cells were loaded with the Ca2+ indicator Fluo-4-AM, and the emission of fluorescence was visualized by confocal microscopy. The Ca2+i concentration was represented as a temporal insensitive graph or as representative pseudocolored images. As shown in Figure 3a, ATP promoted a transient increase in the Ca2+i concentration in both primitive populations. Then, the Ca2+i chelator BAPTA-AM was used to verify the participation of Ca2+i in ATP-induced differentiation. BAPTA-AM was incubated with fresh bone marrow cells for 1 h before stimulus with ATP. The Ca2+i chelator was able to abolish the in vitro ATP-induced differentiation.

Cytokines modulate the observed ATP-induced differentiation

Cytokines and ATP may be released by cells in hematopoietic stem cell niches. Thus, these molecules may regulate HSC activity together. We investigated whether the presence of cytokines could modulate the differentiation induced by ATP. For this purpose, known differentiation effects of ATP were induced to evaluate whether the previous stimulation of cytokines altered the response to ATP.

First, we evaluated whether the addition of cytokines could alter the ATP-dependent increase in the Ca2+i concentration because the effects of ATP-dependent differentiation occur mainly via increased Ca2+i (Figure 3). We observed that a previous 5 min stimulation with cytokines (granulocyte–monocyte colony stimulator factor (GM-CSF), interleukin-3 (IL-3) and stem cell factor (SCF) at 10 ng/ml) partially reduced the ATP-dependent increase in the Ca2+i concentration in LTBMCs. The Ca2+i concentration was represented as a graph of fluorescence intensity versus time or as pseudocolor images. Representative images are shown in Figure 4a and the temporal Ca2+i concentration values are shown in Figure 4b. The increase in Ca2+i was normalized using the Ca2+ ionophore A23187 (Response 100%). A23187 forms membrane channels that allow the passage of Ca2+ to the cytoplasm.

Second, we determined whether cytokines could prevent ATP-dependent differentiation in LTBMCs, as reported previously.6 LTBMCs were incubated with GM-CSF, SCF and IL-3 for 5 min and subsequently stimulated with ATP (1 mM). The cells were collected after 3 days of stimulation, and the percentages of the different populations were analyzed by flow cytometry. Our results showed that the presence of cytokines (10 ng/ml) abolished the increase in the mature myeloid population (Gr-1+Mac-1+) elicited by 1 mM ATP (Figure 5a). The presence of cytokines also inhibited the ATP-dependent decrease in primitive cells (Gr-1Mac-1c-Kit+) in LTBMCs (Figure 5b). In addition, Giemsa/May–Grünwald staining was performed to corroborate the flow cytometry results. The counts of different cell types in the LTBMCs indicated a reduction in the number of the immature and blast forms induced by ATP in LTBMCs, and this effect was inhibited in the presence of cytokines (Table 1).

Finally, we determined whether previous stimulation with cytokines would prevent the ATP-dependent decrease in the HSC reconstitution capacity. Thus, bone marrow cells from GFP+ animals were incubated for 5 min with GM-CSF (10 ng/ml) and IL-3 (10 ng/ml) before stimulation with ATP for 4 h. These cells were then injected into sublethally irradiated animals. After 8 weeks, the bone marrow cells were extracted, and the percentages of GFP+ cells within different hematopoietic populations were calculated. As shown in Figure 6, pretreatment with cytokines followed by 4 h of stimulation with ATP led to a recovery in the reconstitution ability of HSCs. A significant increase in GFP+ cells was observed within the immature population (HSC, HP and MP) and the mature myeloid population (Gr-1+Mac-1+) compared with the group treated with ATP alone.


Discussion

Under physiological conditions, HSCs exhibit a quiescent state and asymmetric proliferation following an interaction with particular osteoblasts in the trabecula bone area. In contrast, myeloid differentiation occurs adjacent to blood vessels.3, 4 In addition, some types of unidentified osteoblast and endothelial cells can release ATP in response to certain stimuli or physiological conditions.8, 9, 10

ATP released by the endothelium or by osteoblasts could participate in myeloid differentiation. In a previous report, we showed that ATP and its analogs could induce the transient proliferation and myeloid differentiation of primitive HPs present in LTBMCs through the activation of P2 receptors.6 In light of these findings, this study was undertaken to investigate the ability of extracellular ATP to induce direct differentiation of HSC and MPs in vivo and in vitro assays.

Initially, we determined the percentage of HSCs and myeloid precursor cells (CMP, GMP and MEP) after stimulation with ATP in freshly isolated bone marrow cells. The percentages of the HSC, CMP and GMP populations present in the bone marrow were reduced by stimulation with ATP, whereas the MEP population remained unchanged (Figure 1a). Subsequently, in vivo experiments showed that treatment with ATP for 4 days led to a reduction in the number of MPs (Figure 1b) and a corresponding increase in the mature myeloid population (Figure 1c). To determine whether the observed changes in blood cell populations were associated with differentiation, we evaluated different HSC characteristics. The presence of a quiescent state, which is a characteristic of HSCs,2 was evaluated. ATP was able to reduce the number of HSCs in quiescent state (Figure 2a). In addition, we investigated whether ATP could alter the expression the receptors Notch, CxCR4 and CaSR. Notch receptors have been associated with the self-renewal, quiescence, maintenance and differentiation of HSCs, and their expression is used as an indicator of the undifferentiated state of HSCs,27, 28, 29 whereas CxCR4 and CaSR are homing receptors required for the colonization of bone marrow by HSCs during the development of hematopoiesis.30, 31 ATP reduced the expression of Notch receptors (Figure 2b). However, there was no observed alteration in the expression of CxCR4 or CaSR (Supplementary Figures 7A and B). Moreover, ATP reduced the ability of HSCs to reconstitute important characteristics of HSC potency in bone marrow (Figure 2c). Therefore, we have showed that ATP reduces the HSC and primitive macrophage/granulocytic progenitor populations, leading to an increase of the mature myeloid population.

Our results showed that the observed ATP-induced differentiation occurred by P2 receptor activation. Participation of the P2 receptor was shown using suramin, a well-known P2 antagonist, in vitro and in vivo experiments (Figure 1). On the other hand, the participation of P1 receptors, activated by adenosine, was excluded by the use of adenosine and xanthine, a P1 receptor inhibitor (Supplementary Figure 5). However, the identification of the P2 receptor subtypes deserves further investigation.

In addition, the maintenance of HSCs in a quiescent state in the osteoblast niche can also be related to a high level of ectonucleotidase expression, including that of CD39 and CD73 (Supplementary Figures 7C–F). Interestingly, primitive hematopoietic cells, characterized as c-Kit+ cells, showed higher ectonucleotidase activity than stromal cells and mature hematopoietic cells (whole bone marrow) (Supplementary Figure 8). These results could explain the lower differentiation effect caused by ATP in vivo assay (Figure 1b). It is possible that the high expression and activity of HSC and stromal cells reduced the differentiation induced by ATP in the hematopoietic niche. Hence, the expression and activity of ectonucleotidases arises like a new important feature to undifferentiated state. However, further investigation on the role of ectonucleotidases in hematopoietic differentiation is needed.

On the other hand, the signaling pathways involved in the differentiation by the activation of P2 receptors have not been completely elucidated. In fact, Ca2+i signaling is the main intracellular pathway triggered by P2 receptors and we have shown its participation in the differentiation induced by ATP. Ca2+ is a ubiquitous messenger that controls many physiological cellular processes in different tissues, including the cells of the bone marrow microenvironment.6, 32 Our results show that ATP induces an increase in Ca2+i in HSC and c-Kit+Lin populations (Figure 3a) and that this differentiation is Ca2+i dependent (Figure 3b). The involvement of Ca2+ in cytokine-mediated signaling was recently reported by our group.33 However, the molecular mechanisms stimulated by Ca2+i to carry out the response to ATP and cytokines remain unknown. The Ca2+i increase elicited by ATP is large and transiently induced in the cells studied here (Figure 3a). In contrast, the change in Ca2+i elicited by cytokines exhibits a short and oscillatory signal.6, 33 Ca2+ signaling stimulated by signaling molecules can influence proliferation, differentiation and cell death, and the response to Ca2+ signaling is determined by proteins that are sensitive to the intensity and duration of the Ca2+ signal.32 It is known that activation of PLCγ, an enzyme that hydrolyzes phosphatidylinositol 4,5-bisphosphate to generate the two second messengers, InsP3 and DAG, and cytosolic Ca2+ modulate several important transcription factor pathways required for the development or differentiation of B cells.34 In myeloid differentiation, the participation of PLCγ2 by the activation of cytokines receptors was described recently.33 Moreover, an unidentified PLCβ is activated by ATP in HSC, which produces InsP3 and DAG similarly to PLCγ. These two PLC isoforms can modulate hematopoiesis depending on the amount of Ca2+i released. Therefore, the increase in Ca2+i levels elicited by ATP or cytokines leads to different cellular effects because distinct Ca2+-sensitive proteins could be activated.

The presence of ATP in hematopoietic niches can be an important factor in regulating HSC activity together with other signaling molecules that regulate hematopoiesis, such as cytokines. Here, we show that the presence of cytokines can modulate ATP-induced differentiation. Previous stimulation of LTBMCs with cytokines reduced the effects of ATP-dependent Ca2+i increases (Figure 4) and hematopoietic differentiation (Figure 5). Finally, previous incubation with cytokines also restored the ability of HSCs to repopulate bone marrow after non-lethal irradiation (Figure 6). Therefore, the intracellular pathways induced by ATP led to differentiation, whereas at the same time, cytokines and other signals activate specific pathways that regulate the maintenance of HPs.

Cytokines activate several intracellular mechanisms that could prevent some overload responses. GM-CSF was able to reduce cell death by activation of chimeric receptor GM-CSF-Bcl-XL protein.35 In addition, moderate elevation of Ca2+i concentration also induces cytokine-independent survival and proliferation of human myelo-erythroid CD34+ leukemia cell line.36. The low increase of Ca2+i concentration by cytokines probably activates the mechanism related with survival decreasing the ATP-induced differentiation.

The results of this study show that extracellular ATP decreases HSC and MPs populations, promoting myeloid differentiation. Moreover, we showed that cytokines are able to modulate ATP-induced differentiation. These findings are important for understanding the HSC niche and point out the importance of the ATP and the P2 receptors in the hematopoiesis.


Materials and Methods
Animals

The wild-type mice (C57BL/6, 8–12 weeks old) used in this study were supplied by the INFAR/UNIFESP Animal Facility (São Paulo, Brazil), and the GFP mice (C57BL/6-Tg(act-EGFP)C14-Y01-FM131Osb) were maintained at CEDEME/UNIFESP. All experiments were approved by the Animal Care and Ethics Committee of the Federal University of São Paulo (0254/08).

In vivo assays

Solutions of nucleotides in saline were prepared as described by Rapaport.26 Briefly, 0.47 g/kg of ATP was diluted in saline (sterile saline, pH 6.2) and intraperitoneally injected daily for 4 days. A control group received an equivalent volume of the vehicle daily (sterile saline) by intraperitoneally injection.25, 26 In some experiments, 10 mg/kg suramin37 and 3 μg/kg xanthine38 were applied 1 h before to ATP injection.

Quantification of hematopoietic populations

Bone marrow cells were extracted from femurs and stimulated with ATP at 37°C for 4 h. All experiments up to 4 h were performed in a physiological solution (137 mM NaCl, 2.68 mM KCl, 1.36 mM CaCl2, 0.49 mM MgCl2, 12 mM NaHCO3, 0.36 mM NaH2PO4, and 5.5 mM -glucose). For experiments more than 12 h long, Iscove's modified Dulbecco's medium (IMDM) supplemented with 2% fetal bovine serum (FBS) was used. Cells were stimulated, and the percentages of each hematopoietic population were determined by flow cytometry and compared with the initial percentage. HP cells were identified as described previously.2, 23 Mature cells were excluded using a lineage marker cocktail (Lin: PE-conjugated B220, CD3, Ter-119, CD11b and Gr-1). In addition, several antibodies (FLK-2-PE, IL-7R-PE, CD90-FITC, Sca-1-Cy7/PE and c-Kit-APC) were used to recognize a subset rich in HSCs (LinFLK-2Sca-1+c-Kit+Thy1.1+ or LinFLK-2Sca-1+c-Kit+); HP (LinSca-1c-Kit+), a population that contained myeloid and lymphoid progenitors; and MP (LinSca-1IL-7c-Kit+). Moreover, CMP (LinIL-7Rc-Kit+Sca-1CD34+FcγRlow), GMP (LinIL-7Rc-Kit+Sca-1CD34+FcγRhigh) and MEP (LinIL-7Rc-Kit+Sca-1CD34FcγRlow) populations were identified using IL-7R-PE, Sca-1-PE, c-Kit-Cy7/PE, CD34-FITC and FCγR-APC antibodies. To quantify the percentage of hematopoietic populations, 3–5 × 106 events were acquired. All antibodies were purchased from Becton Dickinson (Franklin Lakes, NJ, USA) or eBioscience (San Diego, CA, USA). The mature bone marrow-resident myeloid populations were evaluated with antibodies that recognize granulocytic and monocytic populations (Gr-1-PE and CD11b-Cy7/PE, respectively). We captured 3 000 000 events to evaluate this mature population. Data analyses were performed on a FACSCalibur (Becton Dickinson) flow cytometer using the CellQuest software. The gating strategy for murine hematopoietic populations is shown in Supplementary Figure 1.

Murine Linc-Kit+ and CFU-GM assay

Separation of the murine Linc-Kit+ population was performed by gradient centrifugation methods using Ficoll Histopaque (1.077 g/cm3). After mononuclear isolation, the cells were labeled with specific antibodies. For cell isolation, a biotin-conjugated Lin antibody cocktail was used, followed by incubation with anti-biotin-conjugated microbeads (Miltenyi Biotec, Columbus, OH, USA). After labeling, the cells were placed in a magnetic column, and the negative population was extracted. The Lin cells were labeled with anti-c-Kit-conjugated microbeads (Miltenyi Biotec), and the Linc-Kit+ population was isolated in a magnetic column.

CFU assays were performed by plating 5 × 103 murine (Linc-Kit+) bone marrow cells in methylcellulose-based medium with recombinant cytokines and EPO (Methocult M3434; Stem Cell Technologies, Vancouver, Canada) in 35-mm diameter dishes. The cells were cultured in a fully humidified air incubator under 5% CO2 at 37°C for 7 or 14 days for murine and human cells, respectively. At the end of the incubation period, colonies of more than 50 cells were counted using a dark-field microscope.

Notch and Ki-67 expression

Bone marrow cells were stimulated with ATP at 37°C for 2 or 4 h in a physiological solution. Subsequently, cells were fixed with 2% paraformaldehyde for 30 min, washed with 0.1 M glycine and permeabilized with 0.01% saponin for 15 min. The cells were incubated for 2 h with rabbit purified anti-Notch-1 or anti-Ki-67-Alexa Fluor-488 conjugated (Becton Dickinson). Anti-rabbit IgG-Alexa Fluor 488 conjugates (Molecular Probes/Invitrogen, Eugene, OR, USA) was used as secondary antibody against anti-Notch-1 and incubated for 40 min. Notch and Ki-67 expression were evaluated in hematopoietic populations using the antibodies described above. Data analyses were performed on a FACSCalibur flow cytometer using the CellQuest software.

Competitive repopulation assay

Whole bone marrow cells of GFP transgenic mice were stimulated with 1 mM ATP in the presence or absence of GM-CSF (10 ng/ml) and IL-3 (10 ng/ml) in physiological solution at 37°C for 4 h and then injected (106 cells per animal) into wild-type sublethally irradiated mice (700 Gy). The frequency of GFP+ bone marrow cells in each hematopoietic population (HSC, HP, MP and Gr-1+Mac-1+) was quantified at 8 weeks post-transfer using a FACSCalibur flow cytometer.

Dexter-type cultures

Dexter-type cultures or LTBMCs were prepared following the method of Dexter et al.39 with some modifications. Briefly, total bone marrow cells were plated onto 12-well plates and fed weekly with IMDM supplemented with 5% FBS (Cultilab, Rio Grande do Sul, Brazil), 20% horse serum (Stemcell Technologies) and 10−6 M hydrocortisone (Sigma-Aldrich, St Louis, MO, USA). Cultures were maintained at 37°C in a humid chamber with 5% CO2. After the establishment of a confluent stroma layer, the remaining non-adherent cells were removed, and 106 cells per well was added to precultured stroma cultures, along with fresh media. After 1 week of co-culture, the cells were further cultured in IMDM with 0.5% FBS for 24 h. Subsequently, LTMBCs were stimulated with ATP in the presence or absence of IL-3, GM-CSF and SCF. After 3 days, the cells were collected for immunophenotyping using the antibodies Gr-1-FITC, Mac-1-PE and c-Kit-APC. Data analyses were performed on a FACSCalibur flow cytometer (Becton Dickinson). In addition, co-cultured cells were stained with May–Grünwald and Giemsa solution (Sigma) and identified by morphological characteristics.

Calcium measurements by confocal microscopy

Linc-Kit+ and HSC populations were previously isolated to measure Ca2+i. The Lin cells were isolated as described above. HSC isolation was performed by cell sorting in a FACSCalibur flow cytometer.

After the separation of populations, the cells were cytocentrifuged onto glass coverslips (25 mm). For intracellular Ca2+ concentration ([Ca2+]i) measurements, the isolated cells or LTBMCs were incubated for 40 min at room temperature with 10 μM Fluo-4/AM (Molecular Probes/Invitrogen) and 0.01% pluronic acid (Molecular Probes/Invitrogen) and washed with physiological solution. Images were captured with a microscope (Zeiss, Axiovert 100M, Oberkochen, Germany) equipped with a laser scanner (Zeiss, LSM 510 META) and a 63 × objective (Plan-Neofluor, 1.4 numerical aperture) under oil immersion. The Fluo-4 probe was excited with an argon laser (λEx=488 nm), and light emission was detected using a Zeiss META detector (λEm=500–550 nm). The pinhole device was not used for Ca2+i measurements. Images were collected at approximately 4- to 6-s intervals. Fluorescence intensity was normalized with reference to basal fluorescence using the Examiner 3.2 (Zeiss) and Spectralyzer (NJ, USA) software. Representative pseudocolored images are shown with reference to the basal intensity on a fluorescence intensity scale ranging from 0 (black or blue) to 255 (white or red).

Statistical analysis

Ca2+ mobilization in response to ATP was normalized with reference to the response to 10−6 M A23187 (100%), and results were presented as representative pseudocolored images according to a fluorescence intensity scale. Data were expressed as means±S.E.M. Statistical analyses were performed using Student's t-test for comparisons between two groups, and analysis of variance and Dunnett's post hoc test were used for multiple comparisons among groups. Values of P<0.05 were considered statistically significant.


Notes

Supplementary Information accompanies the paper on Cell Death and Disease website (http://www.nature.com/cddis)

Edited by A Stephanou

This work was supported by grants from the ‘Fundação de Amparo à Pesquisa do Estado de São Paulo' (FAPESP). CMVB was supported by a doctoral fellowship from FAPESP (2007/58589-9).


Glossary
BFU-E burst-forming units-erythroid
CaSR calcium-sensing receptors
CFU-GM colony-forming units of granulocytes and macrocytes
CMP common myeloid progenitors
GMPs granulocyte–macrophage progenitors
CxCR4 CxC chemokine receptor 4
DAG diacylglycerol
GM-CSF granulocyte–monocyte colony stimulator factor
HP hematopoietic progenitor
HSCs hematopoietic stem cells
InsP3 inositol 1,4,5-trisphosphate
IL-3 interleukin-3
Ca2+i intracellular Ca2+
Lin lineage marker cocktail
LTBMCs long-term bone marrow cultures
MEPs megakaryocytic–erythroid progenitors
MP myeloid progenitor
PLC phospholipase C
SCF stem cell factor

Notes

The authors declare no conflict of interest.


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Supplementary Material Supplementary Information Click here for additional data file (cddis201149x1.doc)


Article Categories:
  • Original Article

Keywords: ATP, P2 receptors, hematopoietic stem cell, differentiation, myeloid, cytokines.

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