Document Detail

Differentiation and dedifferentiation of the human monocytic leukemia cell line, U937.
MedLine Citation:
PMID:  1282085     Owner:  NLM     Status:  MEDLINE    
U937 cells were differentiated into macrophages after being treated with 12-o-tetradecanoyl-phorbol-13-acetate (TPA) for the first two days and dedifferentiated with daily medium renewal for 10 days. Cell proliferation slowed down and the number of cells reached the maximum level on day 2. By day 4, all of the cells had spread and attached firmly to the culture dish, and more than 90% of the cells expressed the Fc-receptor and produced superoxide anion. From there on, the number of adherent, living cells decreased gradually to about half the initial count. Most of the cells eliminated from the culture by cell death were in the S phase at the time of TPA treatment. After day 8, the number of cells expressing macrophage-specific phenotypes gradually decreased, cell adhesion was weakened, and at the same time, DNA synthesis was initiated anew. The cells became round and began to proliferate as floating cells on days 9 to 10, and thereafter they became sensitive to the second round of TPA treatment. On the basis of all the results taken together, it is suggested that fully differentiated U937 cells were dedifferentiated after being cultured with frequent medium renewal.
H Hosoya; T Marunouchi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell structure and function     Volume:  17     ISSN:  0386-7196     ISO Abbreviation:  Cell Struct. Funct.     Publication Date:  1992 Oct 
Date Detail:
Created Date:  1993-02-01     Completed Date:  1993-02-01     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7608465     Medline TA:  Cell Struct Funct     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  263-9     Citation Subset:  IM    
Division of Cell Biology, Fujita Health University, Aichi, Japan.
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MeSH Terms
Cell Differentiation / drug effects
DNA, Neoplasm / biosynthesis
Leukemia, Myeloid / pathology*
Macrophages / drug effects*
Phagocytosis / physiology
Radioligand Assay
Receptors, Fc / physiology
Staining and Labeling
Tetradecanoylphorbol Acetate / pharmacology*
Tumor Cells, Cultured
Reg. No./Substance:
0/DNA, Neoplasm; 0/Receptors, Fc; 16561-29-8/Tetradecanoylphorbol Acetate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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