| Differentiation is coupled to changes in the cell cycle regulatory apparatus of human embryonic stem cells. | |
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MedLine Citation:
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PMID: 19383386 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mouse embryonic stem cells (mESC) exhibit cell cycle properties entirely distinct from those of somatic cells. Here we investigated the cell cycle characteristics of human embryonic stem cells (hESC). HESC could be sorted into populations based on the expression level of the cell surface stem cell marker GCTM-2. Compared to mESC, a significantly higher proportion of hESC (GCTM-2(+) Oct-4(+) cells) resided in G(1) and retained G(1)-phase-specific hypophosphorylated retinoblastoma protein (pRb). We showed that suppression of traverse through G(1) is sufficient to promote hESC differentiation. Like mESC, hESC expressed cyclin E constitutively, were negative for D-type cyclins, and did not respond to CDK-4 inhibition. By contrast, cyclin A expression was periodic in hESC and coincided with S and G(2)/M phase progression. FGF-2 acted solely to sustain hESC pluripotency rather than to promote cell cycle progression or inhibit apoptosis. Differentiation increased G(1)-phase content, reinstated cyclin D activity, and restored the proliferative response to FGF-2. Treatment with CDK-2 inhibitor delayed hESC in G(1) and S phase, resulting in accumulation of cells with hypophosphorylated pRb, GCTM-2, and Oct-4 and, interestingly, a second pRb(+) GCTM-2(+) subpopulation lacking Oct-4. We discuss evidence for a G(1)-specific, pRb-dependent restriction checkpoint in hESC closely associated with the regulation of pluripotency. |
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Authors:
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Adam A Filipczyk; Andrew L Laslett; Christine Mummery; Martin F Pera |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2007-09-17 |
Journal Detail:
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Title: Stem cell research Volume: 1 ISSN: 1876-7753 ISO Abbreviation: Stem Cell Res Publication Date: 2007 Oct |
Date Detail:
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Created Date: 2009-04-22 Completed Date: 2009-05-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101316957 Medline TA: Stem Cell Res Country: England |
Other Details:
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Languages: eng Pagination: 45-60 Citation Subset: IM |
Affiliation:
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Netherlands Institute for Developmental Biology and Stem Cell Research, Utrecht, The Netherlands. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biological Markers Cell Cycle* Cell Differentiation* Embryonic Stem Cells / cytology G1 Phase Humans Pluripotent Stem Cells / cytology |
| Grant Support | |
ID/Acronym/Agency:
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GM068417-01/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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