Document Detail

Differentiation between vascular permeability factor and IL-2 in lymphocyte supernatants from patients with minimal-change nephrotic syndrome.
MedLine Citation:
PMID:  1914229     Owner:  NLM     Status:  MEDLINE    
Immunotherapy of cancers with recombinant IL-2 induces a vascular leak syndrome which is mainly due to an increase in vascular permeability. A lymphokine, the vascular permeability factor (VPF), which increases vascular permeability, has been characterized in minimal-change nephrotic syndrome (MCNS) and appeared very similar to IL-2. Here we have undertaken a further characterization of VPF in order to determine how closely related this factor was to human IL-2. Both the IL-2 bioassay and Western blot analysis of the MCNS lymphocyte concentrated supernatants with high VPF activity revealed the presence of low quantities of IL-2. Preparative isoelectrofocusing (IEF) of concentrated supernatants resolved each lymphokine in a separate peak, with apparent pIs of 5.2 for VPF and 7.5-10.1 for IL-2. Since a sensitive IL-2 ELISA failed to exhibit any significant antigenic presence of IL-2 in the IEF fractions with the highest VPF activity, we conclude that VPF activity of the concentrated supernatants is not related to IL-2 nor to a biologically inactive form of IL-2. When concentrated supernatants were subjected to preparative SDS-PAGE, VPF activity was recovered within low mol. wt material (1-12 kD). Immunoadsorption experiments gave definite proof since the complete removal of IL-2 from concentrated supernatants did not affect the VPF activity. Although high amounts of IL-2 increased vascular permeability, our experiments clearly demonstrate that VPF is a lymphokine distinct from IL-2.
J M Heslan; A I Branellec; Y Pilatte; P Lang; G Lagrue
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical and experimental immunology     Volume:  86     ISSN:  0009-9104     ISO Abbreviation:  Clin. Exp. Immunol.     Publication Date:  1991 Oct 
Date Detail:
Created Date:  1991-11-14     Completed Date:  1991-11-14     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0057202     Medline TA:  Clin Exp Immunol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  157-62     Citation Subset:  IM    
INSERM U 139, Hôpital Henri Mondor, Créteil, France.
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MeSH Terms
Biological Assay
Blotting, Western
Capillary Permeability
Endothelial Growth Factors / chemistry*
Guinea Pigs
Interleukin-2 / chemistry
Isoelectric Point
Lymphokines / chemistry*
Molecular Weight
Nephrosis, Lipoid / blood,  physiopathology*
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factors
Reg. No./Substance:
0/Endothelial Growth Factors; 0/Interleukin-2; 0/Lymphokines; 0/Vascular Endothelial Growth Factor A; 0/Vascular Endothelial Growth Factors

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