Document Detail


Differentiation between transmissible gastroenteritis virus and porcine respiratory coronavirus using a cDNA probe.
MedLine Citation:
PMID:  1645595     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A plasmid, pG3BS, containing a cDNA clone from the 5' coding region of the peplomer glycoprotein gene appears to be specific for enteric transmissible gastroenteritis virus (TGEV) strains and for live-attenuated TGEV vaccines. This cDNA probe is used to differentiate porcine respiratory coronavirus (PRCV) isolates from TGEV field and vaccine strains by a slot blot hybridization assay. Probe pG3BS also hybridizes to canine coronavirus (CCV) RNA but does not hybridize to antigenically related feline infectious peritonitis virus (FIPV) RNA. The RNAs of 13 enteric TGEV isolates from the United States, Japan, and England, 4 US-licensed live-attenuated TGEV vaccines, and antigenically closely related CCV were detected by pG3BS. The RNAs of FIPV and 3 US isolates of PRCV did not react with pG3BS but were detected by a TGEV-derived plasmid, pRP3. Pigs infected with either PRCV or TGEV test serologically positive for TGEV antibody by the serum neutralization test. Characterization of the virus circulating in a swine herd by the pG3BS probe will differentiate between an enteric TGEV and a respiratory PRCV infection.
Authors:
R D Wesley; I V Wesley; R D Woods
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc     Volume:  3     ISSN:  1040-6387     ISO Abbreviation:  J. Vet. Diagn. Invest.     Publication Date:  1991 Jan 
Date Detail:
Created Date:  1991-07-09     Completed Date:  1991-07-09     Revised Date:  2006-05-01    
Medline Journal Info:
Nlm Unique ID:  9011490     Medline TA:  J Vet Diagn Invest     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  29-32     Citation Subset:  IM    
Affiliation:
USDA, National Animal Disease Center, Ames, IA 50010.
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MeSH Terms
Descriptor/Qualifier:
Animals
Coronaviridae / classification*,  genetics
DNA
DNA Probes*
Nucleic Acid Hybridization
Plasmids
RNA, Viral / analysis*,  genetics
Species Specificity
Transmissible gastroenteritis virus / classification*,  genetics
Viral Vaccines / genetics
Chemical
Reg. No./Substance:
0/DNA Probes; 0/RNA, Viral; 0/Viral Vaccines; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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