Document Detail


Differentiation between carrier-bound forms of non-suppressible insulin-like activity (NSILA) in serum.
MedLine Citation:
PMID:  4065403     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Gel-permeation chromatography of serum on Sephacryl S-300 at pH 7.4 has shown that NSILA was detected over a range of MW 50,000-400,000 with a peak at about MW 200,000. When fractions from the above chromatography were rechromatographed on Sephadex G-75 at pH 2.4 major amounts of acid-stable NSILA were found in a fraction of MW 200,000-600,000 (77% of the fraction NSILA or 28% of total serum NSILA). Further evidence was obtained for the presence of an active acid-dissociable complex in serum. This was present in both the MW 100,000-200,000 and 35,000-100,000 fractions and corresponded to 37% of total serum NSILA. Con-A Sepharose affinity chromatography of the serum fractions from Sephacryl S-300 chromatography, followed by Sephadex G-75 chromatography under acid conditions, showed that the acid-stable complex was consistently found in weakly bound materials. The active acid-dissociable complex was found in the bound fractions, especially in the Sephacryl S-300 pool of MW 35,000-100,000. Low MW NSILA (less than 15,000) was also released on acid treatment from an otherwise inactive high MW complex(es) of MW 35,000-600,000. This complex was not bound by Con-A Sepharose.
Authors:
H J Cornell; M Bistrin; A C Herington
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The International journal of biochemistry     Volume:  17     ISSN:  0020-711X     ISO Abbreviation:  Int. J. Biochem.     Publication Date:  1985  
Date Detail:
Created Date:  1986-01-15     Completed Date:  1986-01-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0250365     Medline TA:  Int J Biochem     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1003-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
Carrier Proteins / isolation & purification*
Chromatography, Affinity / methods
Chromatography, Gel / methods
Humans
Molecular Weight
Nonsuppressible Insulin-Like Activity / isolation & purification*
Reference Values
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/Nonsuppressible Insulin-Like Activity; 0/nonsuppressible insulin-like carrier protein, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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