Document Detail


Differentiation of bacteria using fatty acid profiles from gas chromatography-tandem mass spectrometry.
MedLine Citation:
PMID:  20474059     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Fatty acids are essential components in cell membranes of bacteria, and they determine the different structures of lipids and lipid A. Therefore, fatty acids are good targets for development of rapid and reliable methods for differentiation of bacteria. RESULTS: Here we report fatty acid distributions in three different bacteria-Escherichia coli, Francisella novicida and Bacillus subtilis-studied by using gas chromatography-tandem mass spectrometry. Different fatty acid profiles were observed in the three bacteria. Hydroxyl fatty acids were observed in E. coli and F. novicida, but not in B. subtilis. Carbocyclic fatty acids were observed in E. coli but not in F. novicida or B. subtilis. Methyl-branched fatty acids were observed in B. subtilis but not in E. coli or F. novicida. Although saturated and unsaturated fatty acids were all observed in the three bacteria, their chain lengths and distribution patterns were different. CONCLUSION: The results indicate that the fatty acid profile in a given bacterium is highly specific, and gas chromatography-tandem mass spectrometry is a rapid and sensitive method for the identification or detection of bacteria.
Authors:
Yanyan Li; Shengfang Wu; Liping Wang; Ye Li; Feng Shi; Xiaoyuan Wang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of the science of food and agriculture     Volume:  90     ISSN:  1097-0010     ISO Abbreviation:  J. Sci. Food Agric.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-05-17     Completed Date:  2010-11-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376334     Medline TA:  J Sci Food Agric     Country:  England    
Other Details:
Languages:  eng     Pagination:  1380-3     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2010 Society of Chemical Industry.
Affiliation:
State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, China.
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MeSH Terms
Descriptor/Qualifier:
Bacteria / chemistry*
Bacterial Typing Techniques / methods*
Fatty Acids / analysis*,  classification
Gas Chromatography-Mass Spectrometry / methods
Tandem Mass Spectrometry / methods
Chemical
Reg. No./Substance:
0/Fatty Acids

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