Document Detail

Differentiation-associated genes regulated by TPA-induced c-Jun expression via a PKC/JNK pathway in KYSE450 cells.
MedLine Citation:
PMID:  16480952     Owner:  NLM     Status:  MEDLINE    
A group of potential differentiation-associated genes had been identified by microarray analysis as c-Jun/AP-1 target genes essential for epithelial differentiation program. Our previous study showed that c-Jun/AP-1 could bind and activate these gene promoters in vivo using chromatin immunoprecipitation. To further understand how the mitogen-activated protein kinase signaling pathways regulate AP-1 activity and expression of c-Jun target genes, our strategy was based on the use of 12-o-tetradecanoylophorbol-13-acetate (TPA) and pharmacological reagents to induce or block c-Jun expression. The mRNA and protein expression of these genes increased in response to TPA-induced c-Jun/AP-1 expression. Inhibitors of JNK (SP600125) and PKC (GF109203X) mainly blocked expression and phosphorylation of c-Jun, while inhibition of MEK-ERK activity with PD98059 (an inhibitor of MEK) had little effect. Expression of involucrin and keratin 4 in response to TPA was attenuated by pretreatments with GF109203X and SP600125, but not PD98059, suggesting involvement of PKC and JNK in this response. Taken together, these results suggested that differentiation-associated genes were regulated by TPA-induced c-Jun/AP-1 mainly via a PKC/JNK pathway in esophageal cancer cell line KYSE450.
Xinfeng Yu; Aiping Luo; Changchun Zhou; Fang Ding; Min Wu; Qimin Zhan; Zhihua Liu
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-02-06
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  342     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-02-20     Completed Date:  2006-04-13     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  286-92     Citation Subset:  IM    
National Laboratory of Molecular Oncology, Cancer Institute, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, PR China.
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MeSH Terms
Cell Differentiation* / drug effects
Cell Line, Tumor
DNA / metabolism
Gene Expression Regulation / drug effects*
JNK Mitogen-Activated Protein Kinases / metabolism*
Mitogen-Activated Protein Kinases / metabolism
Phosphorylation / drug effects
Protein Kinase C / metabolism*
Proto-Oncogene Proteins c-jun / metabolism*
Signal Transduction / drug effects*
Tetradecanoylphorbol Acetate / pharmacology*
Transcription Factor AP-1 / metabolism
Transcription, Genetic / drug effects
Reg. No./Substance:
0/Proto-Oncogene Proteins c-jun; 0/Transcription Factor AP-1; 16561-29-8/Tetradecanoylphorbol Acetate; 9007-49-2/DNA; EC Kinase C; EC Mitogen-Activated Protein Kinases; EC Protein Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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