Document Detail


Differentiation of aphid clones by arbitrarily primed polymerase chain reaction (AP-PCR) DNA fingerprinting.
MedLine Citation:
PMID:  8061876     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Differentiation of aphid clones was attempted using AP-PCR which is a simple and rapid method to obtain DNA fingerprints of complex genomes. To establish optimal reaction conditions and examine reproducibility of the method, a laboratory-maintained clone of the pea aphid, Acyrthosiphon pisum, was used as test material. Under the reaction conditions employed, identical fingerprint patterns were obtained throughout a wide range of template DNA amount, from 5 to 800 ng, and irrespective of aphid instar. No changes in the patterns were seen throughout five parthenogenetic generations. When this method was applied to a wild population of the gall-forming aphid, Ceratovacuna nekoashi, five groups of insects originating from different galls formed on the same twig were successfully differentiated from one another by means of polymorphic fingerprint bands. In contrast, the fingerprints of the insects derived from the subgalls of the same gall were identical. These results indicated that in C. nekoashi: (i) members of a gall constitute a clonal population; (ii) a gall is founded by a single fundatrix; and (iii) intergall migration is absent or at least not frequent.
Authors:
T Fukatsu; H Ishikawa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular ecology     Volume:  3     ISSN:  0962-1083     ISO Abbreviation:  Mol. Ecol.     Publication Date:  1994 Jun 
Date Detail:
Created Date:  1994-09-20     Completed Date:  1994-09-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9214478     Medline TA:  Mol Ecol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  187-92     Citation Subset:  IM    
Affiliation:
Zoological Institute, Faculty of Science, University of Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Aphids / classification*,  genetics
Base Sequence
DNA / isolation & purification
DNA Fingerprinting*
DNA Primers
Molecular Sequence Data
Polymerase Chain Reaction / methods*
Templates, Genetic
Chemical
Reg. No./Substance:
0/DNA Primers; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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