Document Detail

Differentiation of adult mouse olfactory precursor cells into hair cells in vitro.
MedLine Citation:
PMID:  17110620     Owner:  NLM     Status:  MEDLINE    
Many forms of deafness result from degeneration of the sensory cells for hearing, the hair cells in the cochlea. Stem cells offer a potential cell-based therapy for the treatment of deafness. Here, we investigate whether adult olfactory precursor cells can differentiate into hair cells in culture. Precursor cells were isolated from mouse olfactory neuroepithelium, were sphere-forming, showed proliferative capacity, and contained cells expressing neuronal and non-neuronal proteins. To induce differentiation, precursor cells were cocultured with cochlear cells and/or cochlear supernatant. Differentiated precursor cells were immunopositive for specific hair cell markers, including myosin VIIa, FM1-43, calretinin, phalloidin, and espin, and resembled hair cells anatomically and immunocytochemically in culture. The results demonstrate for the first time that adult olfactory precursor cells can differentiate into hair cell-like cells, thus providing a potential autotransplantation therapy for hearing loss.
Kharen L Doyle; Anita Kazda; Yvonne Hort; Sarah M McKay; Sharon Oleskevich
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-11-16
Journal Detail:
Title:  Stem cells (Dayton, Ohio)     Volume:  25     ISSN:  1066-5099     ISO Abbreviation:  Stem Cells     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-03-02     Completed Date:  2007-05-29     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9304532     Medline TA:  Stem Cells     Country:  United States    
Other Details:
Languages:  eng     Pagination:  621-7     Citation Subset:  IM    
Neuroscience Research Program, Garvan Institute of Medical Research, 384 Victoria Street, Darlinghurst, Sydney, 2010 New South Wales, Australia.
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MeSH Terms
Cell Differentiation / physiology*
Cells, Cultured
Cochlea / cytology*
Hair Cells, Auditory / cytology*
Mice, Inbred CBA
Olfactory Mucosa / cytology*

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