Document Detail

Differentiation of Streptococcus pneumoniae conjunctivitis outbreak isolates by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
MedLine Citation:
PMID:  18708515     Owner:  NLM     Status:  MEDLINE    
Streptococcus pneumoniae (pneumococcus [Pnc]) is a causative agent of many infectious diseases, including pneumonia, septicemia, otitis media, and conjunctivitis. There have been documented conjunctivitis outbreaks in which nontypeable (NT), nonencapsulated Pnc has been identified as the etiological agent. The use of mass spectrometry to comparatively and differentially analyze protein and peptide profiles of whole-cell microorganisms remains somewhat uncharted. In this report, we discuss a comparative proteomic analysis between NT S. pneumoniae conjunctivitis outbreak strains (cPnc) and other known typeable or NT pneumococcal and streptococcal isolates (including Pnc TIGR4 and R6, Streptococcus oralis, Streptococcus mitis, Streptococcus pseudopneumoniae, and Streptococcus pyogenes) and nonstreptococcal isolates (including Escherichia coli, Enterococcus faecalis, and Staphylococcus aureus) as controls. cPnc cells and controls were grown to mid-log phase, harvested, and subsequently treated with a 10% trifluoroacetic acid-sinapinic acid matrix mixture. Protein and peptide fragments of the whole-cell bacterial isolate-matrix combinations ranging in size from 2 to 14 kDa were evaluated by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Additionally Random Forest analytical tools and dendrogramic representations (Genesis) suggested similarities and clustered the isolates into distinct clonal groups, respectively. Also, a peak list of protein and peptide masses was obtained and compared to a known Pnc protein mass library, in which a peptide common and unique to cPnc isolates was tentatively identified. Information gained from this study will lead to the identification and validation of proteins that are commonly and exclusively expressed in cPnc strains which could potentially be used as a biomarker in the rapid diagnosis of pneumococcal conjunctivitis.
Yulanda M Williamson; Hercules Moura; Adrian R Woolfitt; James L Pirkle; John R Barr; Maria Da Gloria Carvalho; Edwin P Ades; George M Carlone; Jacquelyn S Sampson
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2008-08-15
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  74     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-09-26     Completed Date:  2008-10-23     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5891-7     Citation Subset:  IM    
National Center for Immunizations and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
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MeSH Terms
Bacterial Proteins / analysis
Bacterial Typing Techniques / methods
Cluster Analysis
Conjunctivitis / epidemiology,  microbiology*
Disease Outbreaks*
Enterococcus faecalis / chemistry
Escherichia coli / chemistry
Molecular Epidemiology / methods
Molecular Weight
Pneumococcal Infections / epidemiology,  microbiology*
Proteome / analysis
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
Staphylococcus aureus / chemistry
Streptococcus mitis / chemistry
Streptococcus oralis / chemistry
Streptococcus pneumoniae / chemistry*,  classification*,  isolation & purification
Streptococcus pyogenes / chemistry
Reg. No./Substance:
0/Bacterial Proteins; 0/Proteome

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