Document Detail


Differentiation of Rat Dermal Papilla Cells Into Fibroblast-like Cells Induced by Transforming Growth Factor β1.
MedLine Citation:
PMID:  23149195     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Background:The origin of wound-healing fibroblasts is still debated. Dermal papilla cells (DPCs), which are an important population of stem cells for the regeneration of hair follicles, play a considerable role in cutaneous wound healing. Based on the plasticity of DPCs in wound healing, we hypothesized that DPCs may contribute to the fibroblast population of wound repair.Objective:To explore the possibility of differentiation of DPCs into fibroblasts induced by transforming growth factor β1 (TGF-β1).Methods:The fourth passage DPCs were treated with TGF-β1 (10 ng/mL) for 4 days, and a series of methods was used to observe morphologic changes under an inverted phase contrast microscope, to validate the messenger ribonucleic acid expression change in α-smooth muscle actin (α-SMA) and vimentin by quantitative real-time reverse transcriptase polymerase chain reaction (QRT-PCR), to analyze the expression of α-SMA and vimentin protein by flow cytometry, and to semiquantitatively measure the expression of fibroblast-specific protein 1 (FSP1) by Western blot.Results:DPCs treated with TGF-β1 presented fibroblast-like changes in morphology and immunocytochemistry. The effects of TGF-β1 on α-SMA and vimentin in DPCs were detected on both the transcriptional and the posttranscriptional levels. The results showed that TGF-β1 significantly downregulated α-SMA expression and enhanced the expression of vimentin at all times tested. Further study revealed that TGF-β1 could gradually promote the expression of FSP1 in a time-dependent manner.Conclusion:DPCs experienced the changes in molecular marker expression in response to TGF-β1, which may be a key source of fibroblasts in wound healing.
Authors:
Li Hou-Dong; Shu Bin; Xu Ying-Bin; Shi Yan; Qi Shao-Hai; Li Tian-Zeng; Liu Xu-Sheng; Tang Jin-Ming; Xie Ju-Lin
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Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Journal of cutaneous medicine and surgery     Volume:  16     ISSN:  1203-4754     ISO Abbreviation:  J Cutan Med Surg     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9614685     Medline TA:  J Cutan Med Surg     Country:  -    
Other Details:
Languages:  ENG     Pagination:  400-406     Citation Subset:  -    
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