Document Detail

Differentiation of rat dermal papilla cells into fibroblast-like cells induced by transforming growth factor β1.
MedLine Citation:
PMID:  23149195     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: The origin of wound-healing fibroblasts is still debated. Dermal papilla cells (DPCs), which are an important population of stem cells for the regeneration of hair follicles, play a considerable role in cutaneous wound healing. Based on the plasticity of DPCs in wound healing, we hypothesized that DPCs may contribute to the fibroblast population of wound repair.
OBJECTIVE: To explore the possibility of differentiation of DPCs into fibroblasts induced by transforming growth factor β1 (TGF-β1).
METHODS: The fourth passage DPCs were treated with TGF-β1 (10 ng/mL) for 4 days, and a series of methods was used to observe morphologic changes under an inverted phase contrast microscope, to validate the messenger ribonucleic acid expression change in α-smooth muscle actin (α-SMA) and vimentin by quantitative real-time reverse transcriptase polymerase chain reaction (QRT-PCR), to analyze the expression of α-SMA and vimentin protein by flow cytometry, and to semiquantitatively measure the expression of fibroblast-specific protein 1 (FSP1) by Western blot.
RESULTS: DPCs treated with TGF-β1 presented fibroblast-like changes in morphology and immunocytochemistry. The effects of TGF-β1 on α-SMA and vimentin in DPCs were detected on both the transcriptional and the posttranscriptional levels. The results showed that TGF-β1 significantly downregulated α-SMA expression and enhanced the expression of vimentin at all times tested. Further study revealed that TGF-β1 could gradually promote the expression of FSP1 in a time-dependent manner.
CONCLUSION: DPCs experienced the changes in molecular marker expression in response to TGF-β1, which may be a key source of fibroblasts in wound healing.
Li Hou-dong; Shu Bin; Xu Ying-bin; Shi Yan; Qi Shao-hai; Li Tian-zeng; Liu Xu-sheng; Tang Jin-ming; Xie Ju-lin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cutaneous medicine and surgery     Volume:  16     ISSN:  1203-4754     ISO Abbreviation:  J Cutan Med Surg     Publication Date:    2012 Nov-Dec
Date Detail:
Created Date:  2012-11-15     Completed Date:  2013-04-16     Revised Date:  2014-07-31    
Medline Journal Info:
Nlm Unique ID:  9614685     Medline TA:  J Cutan Med Surg     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  400-6     Citation Subset:  IM    
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MeSH Terms
Actins / metabolism
Calcium-Binding Proteins / metabolism
Cell Differentiation / drug effects*
Cell Proliferation / drug effects
Fibroblasts / cytology,  physiology
Hair Follicle / cytology*
RNA, Messenger / metabolism
Rats, Wistar
Transforming Growth Factor beta1 / pharmacology*
Vimentin / metabolism
Wound Healing / drug effects*
Reg. No./Substance:
0/Actins; 0/Calcium-Binding Proteins; 0/FSP1 protein, rat; 0/RNA, Messenger; 0/Transforming Growth Factor beta1; 0/Vimentin; 0/smooth muscle actin, rat

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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